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GeneCorner plasmid details

Last data update: 19 October 2020 04:30 CEST

Plasmid name: pBRSTd844 (LMBP 1810)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p1810.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Satellite tobacco necrosis virus (STNV); with deletion
Promoter: -
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; use strains with a cI function
Parental clone: pBRSTi844; pPLSTi953
Further information: The plasmid was made of a BglI-NcoI fragment derived from pBRSTi844 (containing the STNV coat protein and the ampicillin gene), and an NcoI-BglI fragment of pPLSTi953 (containing the ORI); in this way, 109 nucleotides are deleted from the 3' UTR region of the STNV genome.
Name mentioned in Van Emmelo et al. (1987) is pSTNVdelI.
Other name of the plasmid is pSTBR1D71.
EMBL Accession number: -
Latest sequence update: 17/09/1989
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr W. Fiers(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Van Emmelo et al., Virology 157 (1987), 480-487 [PMID: 18644559]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 r-m+ (λ)
Host reference: -
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pBRSTd844 (LMBP 1810) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr W. Fiers and was published in Van Emmelo et al., 1987.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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