Last data update: 23 October 2020 04:27 CEST
Plasmid name: pBRSTi163b (LMBP 1808)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Cloned DNA:||Satellite tobacco necrosis virus (STNV); with insertion
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli; use strains with a cI function|
|Further information:||For the construction of this plasmid pBRSTi163a was NcoI opened, filled in and ligated, creating an NsiI (AvaIII) site and repairing the amino acids reading frame; the resulting protein contains 6 additional amino acids.
Other names of the plasmid are pSTBR1L21 and pBRSTNVN164.
|EMBL Accession number:||-|
|Latest sequence update:||26/09/1989|
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by Dr P. Ameloot(1) and Prof. Dr W. Fiers(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 r-m+ (λ)|
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pBRSTi163b (LMBP 1808) is available at BCCM/GeneCorner. This plasmid was deposited by Dr P. Ameloot and Prof. Dr W. Fiers.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.