Last data update: 01 December 2020 04:11 CET
Plasmid name: pBRcHIL6f1 (LMBP 1913)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner core plasmid|
(View with Genome Compiler)
|Cloned DNA:||Human interleukin 6 cDNA (IL6); fragment
|Selection marker:||Tetracycline (tet)|
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli|
|Further information:||The plasmid was constructed by inserting a dT-tailed human interleukin 6 (hIL6) cDNA fragment into the PstI opened, dA-tailed pBR322; this fragment starts at nucleotide 338 of the hIL6 cDNA sequence; so, the coding information for the mature polypeptide is incomplete and the information for the signal peptide is missing.
The length of the natural dA-tail and of the enzymatically added dA/dT-tails has not been determined.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727598.1.
Name mentioned in Haegeman et al. (1986) is 26K-1.
|EMBL Accession number:||LT727598.1, view at EMBL, GenBank, DDBJ|
|Latest sequence update:||10/04/1989|
|Authenticity test:||Restriction enzyme pattern analysed at GeneCorner: AccI and XbaI.|
|History of deposit:||This plasmid was deposited by Prof. Dr G. Haegeman(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Plasmid reference:||Haegeman et al., Eur. J. Biochem. 159 (1986), 625-632 [PMID: 3758081]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli|
|Cultivation medium:||LB-Lennox + tetracycline (10 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pBRcHIL6f1 (LMBP 1913) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr G. Haegeman and was published in Haegeman et al., 1986.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.