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GeneCorner plasmid details

Last data update: 01 December 2020 04:11 CET

Plasmid name: pBRcHIL6f1 (LMBP 1913)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p1913.gb (View with Genome Compiler)
p1913.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human interleukin 6 cDNA (IL6); fragment
Promoter: -
Ribosome
binding site:
-
Terminator: -
Selection marker: Tetracycline (tet)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pBR322
Further information: The plasmid was constructed by inserting a dT-tailed human interleukin 6 (hIL6) cDNA fragment into the PstI opened, dA-tailed pBR322; this fragment starts at nucleotide 338 of the hIL6 cDNA sequence; so, the coding information for the mature polypeptide is incomplete and the information for the signal peptide is missing.
The length of the natural dA-tail and of the enzymatically added dA/dT-tails has not been determined.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727598.1.
Name mentioned in Haegeman et al. (1986) is 26K-1.
EMBL Accession number: LT727598.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 10/04/1989
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: AccI and XbaI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr G. Haegeman(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Haegeman et al., Eur. J. Biochem. 159 (1986), 625-632 [PMID: 3758081]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli
Host reference: -
Cultivation medium: LB-Lennox + tetracycline (10 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pBRcHIL6f1 (LMBP 1913) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr G. Haegeman and was published in Haegeman et al., 1986.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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