Last data update: 24 January 2024 16:39 CET
Plasmid name: pBRcHIL6f1 (LMBP 1913)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p1913.gb
(View with Genome Compiler) p1913.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human interleukin 6 cDNA (IL6, IL-6, IFNB2, BSF2, GeneID 3569); fragment |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Tetracycline (tet) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pBR322 |
| Further information: | The plasmid was constructed by inserting a dT-tailed human interleukin 6 (hIL6) cDNA fragment into the PstI opened, dA-tailed pBR322; this fragment starts at nucleotide 338 of the hIL6 cDNA sequence; so, the coding information for the mature polypeptide is incomplete and the information for the signal peptide is missing. The length of the natural dA-tail and of the enzymatically added dA/dT-tails has not been determined. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727598.1. Name mentioned in Haegeman et al. (1986) is 26K-1. |
| EMBL Accession number: | LT727598.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 10/04/1989 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: AccI and XbaI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr G. Haegeman(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Haegeman et al., Eur. J. Biochem. 159 (1986), 625-632 [PMID: 3758081] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli |
| Host reference: | - |
| Cultivation medium: | LB-Lennox + tetracycline (10 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pBRcHIL6f1 (LMBP 1913) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr G. Haegeman and was published in Haegeman et al., 1986. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.