Last data update: 27 November 2020 04:15 CET
Plasmid name: pBRcHIL6f3 (LMBP 1915)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner core plasmid|
(View with Genome Compiler)
|Cloned DNA:||Human interleukin 6 cDNA (IL6); fragment
|Selection marker:||Tetracycline (tet)|
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli|
|Further information:||The plasmid was constructed by inserting a dT-tailed hIL6 cDNA fragment into the PstI opened, dA-tailed pBR322 vector; this cDNA insert extends 113 bp into the 5' direction (starting at nucleotide 10 of the coding sequence for the mature polypeptide) further than the corresponding insert of pBRcHIL6f2, but lacks 146 bp of the 3' untranslated region preceding the polyA-tail as compared to the cDNA inserts of pBRcHIL6f1 and pBRcHIL6f2.
The length of the enzymatically added dA/dT-tails has not been determined.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727600.1.
Name mentioned in Haegeman et al. (1986) is 26K-7.
|EMBL Accession number:||LT727600.1, view at EMBL, GenBank, DDBJ|
|Latest sequence update:||11/04/1989|
|Authenticity test:||Restriction enzyme pattern analysed at GeneCorner: AccI and XbaI.|
|History of deposit:||This plasmid was deposited by Prof. Dr G. Haegeman(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Plasmid reference:||Haegeman et al., Eur. J. Biochem. 159 (1986), 625-632 [PMID: 3758081]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli|
|Cultivation medium:||LB-Lennox + tetracycline (10 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pBRcHIL6f3 (LMBP 1915) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr G. Haegeman and was published in Haegeman et al., 1986.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.