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GeneCorner plasmid details

Last data update: 27 November 2020 04:15 CET

Plasmid name: pBRcHIL6f3 (LMBP 1915)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p1915.gb (View with Genome Compiler)
p1915.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human interleukin 6 cDNA (IL6); fragment
Promoter: -
Ribosome
binding site:
-
Terminator: -
Selection marker: Tetracycline (tet)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pBR322
Further information: The plasmid was constructed by inserting a dT-tailed hIL6 cDNA fragment into the PstI opened, dA-tailed pBR322 vector; this cDNA insert extends 113 bp into the 5' direction (starting at nucleotide 10 of the coding sequence for the mature polypeptide) further than the corresponding insert of pBRcHIL6f2, but lacks 146 bp of the 3' untranslated region preceding the polyA-tail as compared to the cDNA inserts of pBRcHIL6f1 and pBRcHIL6f2.
The length of the enzymatically added dA/dT-tails has not been determined.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727600.1.
Name mentioned in Haegeman et al. (1986) is 26K-7.
EMBL Accession number: LT727600.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 11/04/1989
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: AccI and XbaI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr G. Haegeman(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Haegeman et al., Eur. J. Biochem. 159 (1986), 625-632 [PMID: 3758081]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli
Host reference: -
Cultivation medium: LB-Lennox + tetracycline (10 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pBRcHIL6f3 (LMBP 1915) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr G. Haegeman and was published in Haegeman et al., 1986.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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