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GeneCorner plasmid details

Last data update: 31 October 2020 04:21 CET

Plasmid name: pBRgHIL61 (LMBP 2989)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p2989.gb (View with Genome Compiler)
p2989.txt
p2989.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human interleukin 6 genomic DNA (IL6, GeneID 3569); exon 1 and 2
Promoter: Human interleukin 6 promoter (IL6)
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Tetracycline (tet)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pBR325; pURgHIL61
Further information: The plasmid was constructed by inserting the EcoRI fragment of pURgHIL61 containing the genomic hIL6 sequence, into the unique EcoRI site of pBR325.
The inserted genomic hIL6 EcoRI fragment contains undetermined upstream intergenic sequences, the 5' flanking region, the promoter as well as the first and second exon and the corresponding intron sequences (the entire first intron and the start of the second intron) of the hIL6 gene.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727419.1.
The nucleotide sequence of the genomic hIL6 DNA corresponds with the EMBL Nucleotide Sequence Database accession number AC073072.11. However, the C nucleotide at promoter position -174 (polymorphism mutant) was changed into a G nucleotide (wild type).
Other name of the plasmid is pBRgHIL6I.
EMBL Accession number: AC073072.11, view at EMBL, GenBank, DDBJ
LT727419.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 03/05/2004
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BamHI, SphI, SspI, StyI, XbaI and XhoI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr G. Haegeman(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Related plasmid reference: Haegeman et al., Eur. J. Biochem. 159 (1986), 625-632 [PMID: 3758081]
Poupart et al., EMBO J. 6 (1987), 1219-1224 [PMID: 3497029]
Yasukawa et al., EMBO J. 6 (1987), 2939-2945 [PMID: 3500852]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml) + tetracycline (10 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pBRgHIL61 (LMBP 2989) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr G. Haegeman.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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