GeneCorner plasmid details

Last data update: 23 July 2021 09:43 CEST

Plasmid name: pBRghIFNGf1 (LMBP 1573)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence:
analysis results


Cloned DNA: Human interferon γ genomic DNA (IFNG); 7.0 kb EcoRI fragment
Promoter: Human interferon γ promoter (IFNG)
binding site:
Terminator: -
Selection marker: Ampicillin (amp)
Tetracycline (tet)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pBR328; λCH4A-gHIIF-2
Further information: The plasmid was constructed by inserting an approx. 7 kb EcoRI fragment from λCH4A-gHIIF-2, containing part of the genomic human IFNγ gene and its 5' flanking region, into the unique EcoRI site of pBR328. The orientation of the inserted fragment is not known.
The nucleotide sequence of the hIFNG gene is a concatenation of two entries obtained from the EMBL Nucleotide Sequence Database accession numbers M32746 and J00219, preceded by an unknown nucleotide sequence fragment of approx. 2440 bp.
The nucleotide sequence of the signal sequence of the hIFNG gene is located from nucleotide 6803 up to and including 6871. As such, the mature hIFNγ is 3 amino acids shorter as compared to the amino acid sequence described in Taya et al. (1982).
Since part of the nucleotide sequence of the 5' flanking region of the hIFNG gene is not known, there is uncertainty as to the cutting frequency of the restriction enzymes indicated.
Other name of the plasmid is pBR328γE7.0.
EMBL Accession number: M32746, view at EMBL, GenBank, DDBJ
Latest sequence update: 30/08/1995
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr W. Fiers(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Taya et al., EMBO J. 1 (1982), 953-958 [PMID: 6329718]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061(λ)
Host reference: Mertens et al., Gene 164 (1995), 9-15 [PMID: 7590329]
Related host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml) + tetracycline (10 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pBRghIFNGf1 (LMBP 1573) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr W. Fiers and was published in Taya et al., 1982.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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