Last data update: 26 July 2021 12:16 CEST
Plasmid name: pBRphoA (LMBP 2954)
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|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner core plasmid|
(View with Genome Compiler)
Escherichia coli alkaline phosphatase A gene (phoA); signal sequence|
|Promoter:||Escherichia coli hybrid tryptophan/lacUV5 promoter (tac)|
|Ribosome binding site (RBS) of the Escherichia coli alkaline phosphatase A gene (phoA)|
|Selection marker:||Ampicillin (amp)
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli|
|Parental clone:||pBR322; pUC19PS1|
|Further information:||This plasmid was constructed by inserting the EcoRI-HindIII fragment from pUC19PS1, containing the Escherichia coli hybrid tryptophan/lacUV5 promoter (tac), the ribosome binding site and the signal sequence of the E. coli alkaline phosphatase A gene (phoA), between the EcoRI and HindIII sites of pBR322.
This plasmid can be used as a secretion vector for bacteria by inserting a gene of interest in phase with the phoA signal sequence.
The presence of the lacI(q) repressor gene is necessary to control the activity of the tac promoter.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727562.1.
|EMBL Accession number:||LT727562.1, view at EMBL, GenBank, DDBJ|
|Latest sequence update:||17/12/1993|
Nucleotide sequence at the end of the phoA signal sequence: * 5' GTG.ACA.AAA.GCG.GTA.CCC.GGG.GAT.CCT.CTA.GAG.TCG.ACC 3' KpnI SmaI XbaI SalI *: End of the signal sequence of phoA. Punctuation indicates reading frame.
|Authenticity test:||Restriction enzyme pattern analysed at GeneCorner: PstI, SalI and XbaI.|
|History of deposit:||This plasmid was deposited by Dr K. De Sutter(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Plasmid reference:||Flamez et al., J. Biotechnol. 42 (1995), 133-143 [PMID: 7576533]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 DH5α|
|Host reference:||Focus 8 (1986), 9
|Related host reference:||Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml) + tetracycline (10 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pBRphoA (LMBP 2954) is available at BCCM/GeneCorner. This plasmid was deposited by Dr K. De Sutter and was published in Flamez et al., 1995.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.