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GeneCorner plasmid details

Last data update: 05 December 2020 04:15 CET

Plasmid name: pBRphoAE6H (LMBP 2956)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p2956.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Escherichia coli alkaline phosphatase A gene (phoA); signal sequence
Mouse anti-hPLAP monoclonal antibody E6(IgG2b,κ) cDNA; heavy chain (E6H) missing the leader signal sequence
Promoter: Escherichia coli hybrid tryptophan/lacUV5 promoter (tac)
Ribosome
binding site:
Ribosome binding site (RBS) of the Escherichia coli alkaline phosphatase A gene (phoA)
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pBRphoA; pSV23SE6Hm
Further information: The plasmid was constructed by inserting the KpnI-SalI fragment of pSV23SE6Hm (containing E6H gene, without the leader signal sequence) between the KpnI and SalI sites of pBRphoA. Both KpnI sites were previously blunted with T4 (exonuclease), which connects the fusion in phase.
The presence of the lacI(q) repressor gene is necessary to control the activity of the tac promoter.
EMBL Accession number: -
Latest sequence update: 01/02/1994
Sequence detail:
Nucleotide sequence of the sphoA-E6H fusion:


       sphoA  -->|<--  VH
                 |
5' ...ACA AAA GCG CAG GTT CAG CTG... 3'
      Thr Lys Ala Gln Val Gln Leu
                          PvuII
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr K. De Sutter(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Flamez et al., J. Biotechnol. 42 (1995), 133-143 [PMID: 7576533]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061F'
Host reference: -
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml) + tetracycline (10 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pBRphoAE6H (LMBP 2956) is available at BCCM/GeneCorner. This plasmid was deposited by Dr K. De Sutter and was published in Flamez et al., 1995.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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