Last data update: 24 January 2024 16:39 CET
Plasmid name: pBRphoAE6L (LMBP 2959)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p2959.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Escherichia coli alkaline phosphatase A gene (phoA); signal sequence Mouse anti-hPLAP monoclonal antibody E6(IgG2b,κ) cDNA; light chain (E6L) missing the leader signal sequence |
| Promoter: | Escherichia coli hybrid tryptophan/lacUV5 promoter (tac) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli alkaline phosphatase A gene (phoA) |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pBRphoA; pSV23SE6Lm |
| Further information: | The plasmid was constructed by inserting the BsmI-SalI fragment of pSV23SE6Lm (containing E6L without the leader signal sequence) between the KpnI and SalI sites of pBRphoA. Both KpnI and BsmI sites were previously blunted with T4 (exonuclease), which makes the fusion in phase. The presence of the lacI(q) repressor gene is necessary to control the activity of the tac promoter. |
| EMBL Accession number: | - |
| Latest sequence update: | 04/02/1994 |
| Sequence detail: | Nucleotide sequence of the sphoA-E6L fusion:
sphoA -->|<-- VL
|
5' ...ACA AAA GCG GAC ATC CAG... 3'
Thr Lys Ala Asp Ile Gln |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr K. De Sutter(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Flamez et al., J. Biotechnol. 42 (1995), 133-143 [PMID: 7576533] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061F' |
| Host reference: | - |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) + tetracycline (10 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pBRphoAE6L (LMBP 2959) is available at BCCM/GeneCorner. This plasmid was deposited by Dr K. De Sutter and was published in Flamez et al., 1995. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.