Last data update: 18 January 2021 04:24 CET
Plasmid name: pBRsE6Hf1sE6L (LMBP 2961)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
Escherichia coli alkaline phosphatase A gene (phoA); signal sequence|
Mouse anti-hPLAP monoclonal antibody E6(IgG2b,κ) cDNA; truncated heavy chain of the Fab fragment E6F1 (E6Hf1)
Mouse anti-hPLAP monoclonal antibody E6(IgG2b,κ) cDNA; light chain (E6L) missing the leader signal sequence
|Promoter:||Escherichia coli hybrid tryptophan/lacUV5 promoter (tac)|
|Ribosome binding site (RBS) of the Escherichia coli alkaline phosphatase A gene (phoA)|
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli|
|Parental clone:||pBRphoAE6Hf1; pBRphoAE6L|
|Further information:||This plasmid was constructed by inserting the XhoI-NruI fragment of pBRphoAE6L (containing the tac promoter and the sphoA-E6L cassette) between the SalI and NruI sites of pBRphoAE6Hf1.
The plasmid is used for expression of the Fab fragment of mouse anti-hPLAP antibody E6.
The presence of the lacI(q) repressor gene is necessary to control the activity of the tac promoters.
Name mentioned in Flamez et al. (1995) is pBRphoAFab.
Other name of the plasmid is pBRphoAE6Fab.
|EMBL Accession number:||-|
|Latest sequence update:||08/02/1994|
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by Dr K. De Sutter(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Plasmid reference:||Flamez et al., J. Biotechnol. 42 (1995), 133-143 [PMID: 7576533]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 MC1061F'|
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml) + tetracycline (10 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pBRsE6Hf1sE6L (LMBP 2961) is available at BCCM/GeneCorner. This plasmid was deposited by Dr K. De Sutter and was published in Flamez et al., 1995.|