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GeneCorner plasmid details

Last data update: 26 October 2020 04:25 CET

Plasmid name: pBRsE6Hf2sE6L (LMBP 2962)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p2962.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Escherichia coli alkaline phosphatase A gene (phoA); signal sequence
Mouse anti-hPLAP monoclonal antibody E6(IgG2b,κ) cDNA; truncated heavy chain of the F(ab')2 fragment E6F2 (E6Hf2)
Mouse anti-hPLAP monoclonal antibody E6(IgG2b,κ) cDNA; light chain (E6L) missing the leader signal sequence
Promoter: Escherichia coli hybrid tryptophan/lacUV5 promoter (tac)
Ribosome
binding site:
Ribosome binding site (RBS) of the Escherichia coli alkaline phosphatase A gene (phoA)
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pBRphoAE6Hf2; pBRphoAE6L
Further information: This plasmid was constructed by inserting the XhoI-NruI fragment of pBRphoAE6L (containing the tac promoter and the sphoA-E6L cassette) between the SalI and NruI sites of pBRphoAE6Hf2.
The plasmid is used for expression of the F(ab')2 fragment of the mouse anti-hPLAP antibody E6.
The presence of the lacI(q) repressor gene is necessary to control the activity of the tac promoter.
Name mentioned in Flamez et al. (1995) is pBRphoAF(ab')2.
Other name of the plasmid is pBRphoAE6F(ab')2.
EMBL Accession number: -
Latest sequence update: 10/02/1994
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr K. De Sutter(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Flamez et al., J. Biotechnol. 42 (1995), 133-143 [PMID: 7576533]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061F'
Host reference: -
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml) + tetracycline (10 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pBRsE6Hf2sE6L (LMBP 2962) is available at BCCM/GeneCorner. This plasmid was deposited by Dr K. De Sutter and was published in Flamez et al., 1995.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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