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GeneCorner plasmid details

Last data update: 26 October 2020 04:25 CET

Plasmid name: pBRsE6Hy3 (LMBP 2966)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p2966.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Escherichia coli alkaline phosphatase A gene (phoA); signal sequence
Mouse anti-hPLAP monoclonal antibody E6(Hy3,κ) cDNA; mouse::human chimeric heavy chain (E6Hy3)
Promoter: Escherichia coli hybrid tryptophan/lacUV5 promoter (tac)
Ribosome
binding site:
Ribosome binding site (RBS) of the Escherichia coli alkaline phosphatase A gene (phoA)
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pBRphoAE6Hf2; pSV51cE6Hy3
Further information: The plasmid was constructed by ligation of three fragments: 1) the large EcoRI-SalI fragment (containing ori and amp) of pBRphoAE6Hf2; 2) the EcoRI-ApaLI fragment (containing tac promoter, signal sequence of phoA, the variable domain and a fragment of the constant domain 1 of E6H) of pBRphoAE6Hf2; 3) the ApaLI-SalI fragment of pSV51cE6Hy3 (containing the completing part of constant domain 1, the hinge region and the constant domains 2 and 3 of hIG3).
The sphoA-E6Hy3 fusion is in phase.
The presence of the lacI(q) repressor gene is necessary to control the activity of the tac promoter.
Name mentioned in Flamez et al. (1995) is pBRphoAcHy3H.
EMBL Accession number: -
Latest sequence update: 23/01/1997
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr K. De Sutter(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Flamez et al., J. Biotechnol. 42 (1995), 133-143 [PMID: 7576533]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061F'
Host reference: -
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml) + tetracycline (10 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pBRsE6Hy3 (LMBP 2966) is available at BCCM/GeneCorner. This plasmid was deposited by Dr K. De Sutter and was published in Flamez et al., 1995.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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