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GeneCorner plasmid details

Last data update: 27 November 2020 04:15 CET

Plasmid name: pBRsE6Hy3f2 (LMBP 2965)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p2965.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Escherichia coli alkaline phosphatase A gene (phoA); signal sequence
Mouse anti-hPLAP monoclonal antibody E6(Hy3,κ) cDNA; truncated mouse::human chimeric heavy chain of the F(ab')2 fragment E6(Hy3,κ)F2 (E6Hy3f2)
Promoter: Escherichia coli hybrid tryptophan/lacUV5 promoter (tac)
Ribosome
binding site:
Ribosome binding site (RBS) of the Escherichia coli alkaline phosphatase A gene (phoA)
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pBRphoAE6Hf2; pSV51cE6Hy3f2
Further information: The plasmid was constructed by ligation of three fragments; the large EcoRI-SalI fragment (ori and amp) and the EcoRI-ApaLI fragment (tac promoter, signal sequence of phoA, the variable domain and a fragment of the constant domain of E6H) of pBRphoAE6Hf2, with the ApaLI-SalI fragment of pSV51cE6Hy3f2 (containing part of the constant domain 1, the hinge and constant domain 2 of the cDNA of hIG3).
This plasmid is similar to pBRsE6Hy3, but the cDNA encoding the mouse::human chimeric heavy chain of E6(Hy3,κ) is not complete. After translation, E6Hy3f2 contains the first 27 amino acids of the original CH2 domain followed by 4 amino acids derived from the cloning strategy and the multi-stop-linker.
The sphoA-E6Hy3f2 fusion is in-phase.
The presence of the lacI(q) repressor gene is necessary to control the activity of the tac promoter.
Name mentioned in Flamez et al. (1995) is pBRphoAcHy3f2.
EMBL Accession number: -
Latest sequence update: 23/01/1997
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr K. De Sutter(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Flamez et al., J. Biotechnol. 42 (1995), 133-143 [PMID: 7576533]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061F'
Host reference: -
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml) + tetracycline (10 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pBRsE6Hy3f2 (LMBP 2965) is available at BCCM/GeneCorner. This plasmid was deposited by Dr K. De Sutter and was published in Flamez et al., 1995.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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