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GeneCorner plasmid details

Last data update: 19 October 2020 04:30 CEST

Plasmid name: pBT16 (LMBP 9600)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p9600.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Saccharomyces cerevisiae serine/threonine protein kinase 1 cDNA (YPK1); mutated sequence
Influenza HA epitope encoding the haemagglutinin tagging peptide; N-terminal
Promoter: Saccharomyces cerevisiae serine/threonine protein kinase 1 promoter (YPK1)
Phage T3 promoter
Phage T7 gene 10 promoter (T7g10)
Ribosome
binding site:
-
Terminator: Saccharomyces cerevisiae serine/threonine protein kinase 1 terminator (YPK1)
Selection marker: Ampicillin (amp)
Saccharomyces cerevisiae URA3; auxotrophic
Replicon: Escherichia coli plasmid pMB1 origin
Saccharomyces cerevisiae centromeric region 6 (CEN6)/ S. cerevisiae autonomously replicating sequence ARSH4
Phage f1 origin
Host range: Escherichia coli
Saccharomyces cerevisiae; ura3(-)
Parental clone: pSR101; pPL220
Further information: The plasmid was constructed by amplifying the mutated S. cerevisiae YPK1 coding sequence from pPL220 and cloning it in the ClaI/MfeI opened pSR101 plasmid.
The S. cerevisiae YPK1 coding sequence contains an L424G mutation.
The nucleotide sequence of the wild type S. cerevisiae YPK1 cDNA, YPK1 promoter and YPK1 terminator corresponds with the EMBL Nucleotide Sequence Database accession number BK006944.2.
EMBL Accession number: BK006944.2, view at EMBL, GenBank, DDBJ
Latest sequence update: 05/08/2015
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr S. Moye-Rowley (1). It was constructed by B. Thommandru (1).
(1) Laboratory of Molecular and cellular biology of eukaryotic stress resistance, Department of Molecular Physiology and Biophysics, Carver College of Medicine, University of Iowa, Iowa City, USA
Plasmid reference: Khakhina et al., Eukaryotic Cell 14 (2015), 442-453 [PMID: 25724885] [DOI: 10.1128/EC.00021-15]
Restricted distribution: - BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pBT16 (LMBP 9600) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr S. Moye-Rowley and was published in Khakhina et al., 2015.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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