Last data update: 22 October 2020 04:25 CEST
Plasmid name: pBW4 (LMBP 4112)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner core plasmid|
(View with Genome Compiler)
|Cloned DNA:||Chloramphenicol acetyltransferase gene (cat)
RNA one modulator gene (rom)
|Selection marker:||Chloramphenicol (cam)|
|Replicon:||Enterobacter agglomerans plasmid pPIGDM1 origin|
|Host range:||Escherichia coli
|Parental clone:||pPIGDM1; pBR328|
|Further information:||This plasmid was constructed by inserting a HindII fragment of pBR328, containing the chloramphenicol resistance gene, into the unique HindII site of pPIGDM1.
pBW4 carries the rom gene, regulating the copy number of the plasmid (10 to 12 in E. coli K12 MG1655).
pBW4 can be used for the construction of cloning vectors compatible with ColE1/pMB1-, p15A-, RSF1030- or CloDF13-derived plasmids.
Dr B. Wrobel communicated the presence of an unexpected HindIII site near the HindII junction.
Sequence analysis, performed at LMBP, revealed the presence of an additional 432 bp HindII fragment of unknown origin, containing the unexpected HindIII site.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727305.1.
The nucleotide sequence of the pPIGDM1-derived part of the plasmid corresponds with the EMBL Nucleotide Sequence Database accession number AF014880.1.
The nucleotide sequence of the inserted HindII fragment of pBR328 corresponds with the EMBL Nucleotide Sequence Database accession number L08858.1.
|EMBL Accession number:||AF014880.1, view at EMBL, GenBank, DDBJ
L08858.1, view at EMBL, GenBank, DDBJ
LT727305.1, view at EMBL, GenBank, DDBJ
|Latest sequence update:||20/11/2001|
|Authenticity test:||Restriction enzyme pattern analysed at GeneCorner: EcoRI, EcoRV/XhoI, HindII and HindIII.|
|History of deposit:||This plasmid was deposited by Dr B. Wrobel(1).
(1) Department of Molecular Biology, University of Gdansk, Poland
|Plasmid reference:||Mikiewicz et al., Plasmid 38 (1997), 210-219 [PMID: 9435023]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 MG1655|
|Host reference:||Neidhardt et al. (eds), Escherichia coli and Salmonella: Cellular and Molecular Biology, American Society for Microbiology, Washington DC (1996) [ISSN/ISBN: 1555810845]
Blattner et al., Science 277 (1997), 1453-1462 [PMID: 9278503]
|Cultivation medium:||LB-Lennox + chloramphenicol (25 μg/ml)|
|Other culture collection numbers:||NIG-NBRP ?|
Refer in your Materials and Methods:
|pBW4 (LMBP 4112) is available at BCCM/GeneCorner. This plasmid was deposited by Dr B. Wrobel and was published in Mikiewicz et al., 1997.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.