Last data update: 24 January 2024 16:39 CET
Plasmid name: pBact-Pcad (LMBP 2766)
| New search | Print data sheet |
| Price category: | Cat. 2 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p2766.gb
(View with Genome Compiler) p2766.txt p2766.pdf |
| Sequence analysis results Genecorner: |
Sanger: 64420.fasta Sanger: 64421.fasta Sanger: 67921.fasta Sanger: 67922.fasta Sanger: 67923.fasta Sanger: 67924.fasta Sanger: 67925.fasta |
| Cloned DNA: |
Mouse cadherin 3 cDNA (Cdh3, Cadp, P-cadherin, Pcad, Cdhp, GeneID 12560) |
| Promoter: | Chicken β-actin promoter (ACTB) Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli Mammalian cells |
| Parental clone: | pβact-CAT9; P-cad/BLSC |
| Further information: | The plasmid was constructed by isolating the mouse Cdh3 coding sequence with its flanking UTRs from P-cad/BLSC as a HindIII-SmaI fragment, and cloning it into the HindIII-HpaI opened pβact-CAT9 vector. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727542.1 except for some changes in the 5' UTR and 3' UTR of mouse Cdh3. The nucleotide sequence of the mouse Cdh3 cDNA corresponds with the Genbank accession number NM_001037809.5. The nucleotide sequence of the 3' part of the ACTB promoter region and of the 5' UTR of the chicken β-actin gene correspond with the EMBL Nucleotide Sequence Database accession number X00182.1 Name mentioned in Nose et al. (1988) is pβact-Pcad. |
| EMBL Accession number: | NM_001037809.5, view at GenBank X00182.1, view at EMBL, GenBank, DDBJ LT727542.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 30/03/2022 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BglI, HindIII, HindIII/SmaI, KpnI, SacI and SmaI. Part of the ACTB promoter and the entire mouse Cdh3 sequence were sequenced at BCCM/GeneCorner. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr M. Takeichi(1). (1) Faculty of Science, Department of Biophysics, Kyoto University, Japan |
| Plasmid reference: | Nose et al., Cell 54 (1988), 993-1001 [PMID: 3416359] |
| Related plasmid reference: | Cachat et al., Sci. Rep. 6 (2016), 20664 [PMID: 26857385] [DOI: 10.1038/srep20664] Nose et al., EMBO J. 6 (1987), 3655-3661 [PMID: 3428270] Fregien et al., Gene 48 (1986), 1-11 [PMID: 3470237] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pBact-Pcad (LMBP 2766) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr M. Takeichi and was published in Nose et al., 1988. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.