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GeneCorner plasmid details

Last data update: 23 October 2020 04:27 CEST

Plasmid name: pBact-Pcad (LMBP 2766)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p2766.gb (View with Genome Compiler)
p2766.txt
p2766.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: Mouse cadherin 3 cDNA (Cdh3, Cadp, P-cadherin, Pcad)
Promoter: Chicken β-actin promoter (Ac)
Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Mammalian cells
Parental clone: pβact-CAT9; P-cad/BLSC
Further information: The plasmid contains the mCdh3 cDNA, inserted as a HindIII-SmaI fragment from the plasmid P-cad/BLSC into the HindIII-HpaI opened pβact-CAT9.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727542.1.
Since the nucleotide sequence at the 5' end of the insert could not be traced back, there is uncertainty as to the cutting frequency of the restriction enzymes, except for the sites that were analysed in the authenticity test.
The 5' half of the Ac promoter, the mouse Cdh3 coding sequence and the 3' UTR of the mouse Cdh3 cDNA were sequenced by LMBP. The Cdh3 sequence analysis results confirmed that the nucleotide sequence of the mouse Cdh3 cDNA corresponds with the Genbank accession number NM_001037809.4.
The nucleotide sequence of the 3' part of the Ac promoter region and of the 5' UTR of the chicken β-actin gene correspond with the EMBL Nucleotide Sequence Database accession number X00182.1
Name mentioned in Nose et al. (1988) is pβact-Pcad.
EMBL Accession number: NM_001037809.4, view at GenBank
X00182.1, view at EMBL, GenBank, DDBJ
LT727542.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 23/11/2010
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BglI, HindIII, HindIII/SmaI, KpnI, SacI and SmaI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr M. Takeichi(1).
(1) Faculty of Science, Department of Biophysics, Kyoto University, Japan
Plasmid reference: Nose et al., Cell 54 (1988), 993-1001 [PMID: 3416359]
Related plasmid reference: Fregien et al., Gene 48 (1986), 1-11 [PMID: 3470237]
Nose et al., EMBO J. 6 (1987), 3655-3661 [PMID: 3428270]
Cachat et al., Sci Rep 6 (2016), 20664 [PMID: 26857385] [DOI: 10.1038/srep20664]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pBact-Pcad (LMBP 2766) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr M. Takeichi and was published in Nose et al., 1988.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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