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GeneCorner plasmid details

Last data update: 27 October 2020 04:27 CET

Plasmid name: pBluMGAPDHf (LMBP 2800)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p2800.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Mouse glyceraldehyde 3 phosphate dehydrogenase cDNA (mGAPDHf); fragment
Promoter: Phage T7 gene 10 promoter (T7g10)
Phage T3 promoter
Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Host range: Escherichia coli
Parental clone: pBluescriptIISK-
Further information: This plasmid is constructed by inserting a mouse GAPDH fragment (clone 2G61F from P19EC library of L. Jonk) provided with an EcoRI/SmaI adaptor (GAATTCCCGGG) in the unique EcoRI site of the polylinker of pBluescriptIIKS-.
GAPDH is a 'house keeping' gene and can be used as hybridisation control.
The inserted fragment is homologous with residue 115-519 of rat GAPDH cDNA. It contains 41 nucleotides of the 5' UTR region and the first 436 nucleotides of the mGADPH coding sequence.
When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide.
EMBL Accession number: -
Latest sequence update: 08/04/1997
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr L.J.C. Jonk(1).
(1) Biologisch Centrum, Haren, The Netherlands
Plasmid reference: Jonk et al., Mech. Dev. 36 (1992), 165-172 [PMID: 1315151]
Related plasmid reference: Fort et al., Nucleic Acids Res. 13 (1985), 1413-1442 [PMID: 2987824]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pBluMGAPDHf (LMBP 2800) is available at BCCM/GeneCorner. This plasmid was deposited by Dr L.J.C. Jonk and was published in Jonk et al., 1992.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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