Last data update: 27 October 2020 04:27 CET
Plasmid name: pBluMGAPDHf (LMBP 2800)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Cloned DNA:||Mouse glyceraldehyde 3 phosphate dehydrogenase cDNA (mGAPDHf); fragment
|Promoter:||Phage T7 gene 10 promoter (T7g10)
Phage T3 promoter
Escherichia coli lac operon promoter
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin
Phage f1 origin
|Host range:||Escherichia coli|
|Further information:||This plasmid is constructed by inserting a mouse GAPDH fragment (clone 2G61F from P19EC library of L. Jonk) provided with an EcoRI/SmaI adaptor (GAATTCCCGGG) in the unique EcoRI site of the polylinker of pBluescriptIIKS-.
GAPDH is a 'house keeping' gene and can be used as hybridisation control.
The inserted fragment is homologous with residue 115-519 of rat GAPDH cDNA. It contains 41 nucleotides of the 5' UTR region and the first 436 nucleotides of the mGADPH coding sequence.
When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide.
|EMBL Accession number:||-|
|Latest sequence update:||08/04/1997|
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by Dr L.J.C. Jonk(1).
(1) Biologisch Centrum, Haren, The Netherlands
|Plasmid reference:||Jonk et al., Mech. Dev. 36 (1992), 165-172 [PMID: 1315151]
|Related plasmid reference:||Fort et al., Nucleic Acids Res. 13 (1985), 1413-1442 [PMID: 2987824]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 MC1061|
|Host reference:||Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
|Related host reference:||Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pBluMGAPDHf (LMBP 2800) is available at BCCM/GeneCorner. This plasmid was deposited by Dr L.J.C. Jonk and was published in Jonk et al., 1992.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.