GeneCorner plasmid details

Last data update: 17 June 2021 09:10 CEST

Plasmid name: pBluMTNFR55 (LMBP 2559)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: (View with Genome Compiler)
analysis results


Cloned DNA: Mouse tumor necrosis factor receptor p55 cDNA (TNFR55, TNFR1, TNFRSF1A)
Promoter: Escherichia coli lac operon promoter
Phage T7 gene 10 promoter (T7g10)
Phage T3 promoter
binding site:
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Host range: Escherichia coli
Parental clone: pBluescriptIIKS-
Further information: The plasmid was constructed by inserting mTNFR55 cDNA as an EcoRI fragment into the unique EcoRI site of pBluescriptIIKS-, in the antisense orientation relative to the T7 promoter.
When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727528.1.
Other name of the plasmid is pBlMTNFR55.
EMBL Accession number: LT727528.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 21/06/1993
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: AccB7I, AccB7I/NarI, ApaI, EcoRI, NarI and ScaI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by F. Hoffmann - La Roche LTD(1).
(1) Basel, Switserland
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pBluMTNFR55 (LMBP 2559) is available at BCCM/GeneCorner. This plasmid was deposited by F. Hoffmann - La Roche LTD.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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