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GeneCorner plasmid details

Last data update: 24 October 2020 04:12 CEST

Plasmid name: pBlueBacmsGCB1-H105F (LMBP 4643)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p4643.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Mouse guanylate cyclase 1, soluble, beta 3 cDNA (Gucy1b3, beta 1 sGC, sGCB1); mutated sequence
Promoter: Autographa californica nuclear polyhedrosis virus (AcNPV) polyhedrin promoter (PH)
Autographa californica nuclear polyhedrosis virus (AcNPV) EcoRI-T large promoter (ETL)
Ribosome
binding site:
-
Terminator: Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Insect cells; e.g. Sf9 cells
Parental clone: pBlueBacmsGCB1
Further information: The plasmid was constructed via site-directed mutagenesis of pBlueBacmsGCB1, introducing a H105F mutation and a unique BalI restriction site.
Other name of the plasmid is pBlueBac4.5/V5-msGCBeta_H105F.
EMBL Accession number: -
Latest sequence update: 12/12/2002
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BglII, EcoRV, HaeII and HindIII/SacI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr P. Brouckaert(1) (2). It was constructed by M. Buys(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 TOP10
Host reference: Invitrogen Instruction Manual
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pBlueBacmsGCB1-H105F (LMBP 4643) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr P. Brouckaert .

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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