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GeneCorner plasmid details

Last data update: 30 October 2020 04:11 CET

Plasmid name: pBluescriptIISK(-)-IRES-LacZ (LMBP 7845)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p7845.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Escherichia coli lac Z gene (lacZ)
Promoter: Phage T7 gene 10 promoter (T7g10)
Phage T3 promoter
Escherichia coli lac operon promoter
Ribosome
binding site:
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ)
Internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV) polyprotein gene
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Host range: Escherichia coli; use strains expressing the lacZ ω fragment (lacZΔM15) for screening
Parental clone: pBluescriptIISK(-)
Further information: The nucleotide sequences of the lacZ CDS and the ECMV IRES correspond with the EMBL Nucleotide Sequence Database accession number X52330.1.
The region downstream from the T7 promoter and the region upstream from the T3 promoter were sequenced by LMBP.
Other name of the plasmid is pBluescriptIISK- IRES-LacZ.
EMBL Accession number: X52330.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 10/09/2012
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr R. Devos(1).
(1) Roche Research Gent, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pBluescriptIISK(-)-IRES-LacZ (LMBP 7845) is available at BCCM/GeneCorner. This plasmid was deposited by Dr R. Devos.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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