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GeneCorner plasmid details

Last data update: 01 December 2020 04:11 CET

Plasmid name: pBluescriptIISK(-)-rob (LMBP 7839)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p7839.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Rattus norvegicus leptin cDNA (LEP, OB)
Promoter: Phage T7 gene 10 promoter (T7g10)
Phage T3 promoter
Escherichia coli lac operon promoter
Ribosome
binding site:
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ)
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Host range: Escherichia coli; use strains expressing the lacZ ω fragment (lacZΔM15) for screening
Parental clone: pBluescriptIISK(-)
Further information: The nucleotide sequence of the rat LEP cds and its 5' and 3' untranslated regions corresponds with the Genbank accession number NM_013076.3.
The region between the T7 promoter and the T3 promoter was sequenced by LMBP.
Other name of the plasmid is pBluescript SK(-) Rat ob (cDNA).
EMBL Accession number: NM_013076.3, view at GenBank
Latest sequence update: 10/09/2012
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: AvaI, BglI, EcoRI/XhoI and SacI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr R. Devos(1).
(1) Roche Research Gent, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pBluescriptIISK(-)-rob (LMBP 7839) is available at BCCM/GeneCorner. This plasmid was deposited by Dr R. Devos.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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