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GeneCorner plasmid details

Last data update: 27 October 2020 04:27 CET

Plasmid name: pBluhANCTNf (LMBP 2911)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p2911.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human αN-catenin cDNA (aNctn, CAP-R, CTNNA2, catna2); fragment
Promoter: Phage T7 gene 10 promoter (T7g10)
Phage T3 promoter
Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Host range: Escherichia coli
Parental clone: pBluescriptIISK-
Further information: The plasmid was constructed by inserting a 1850 bp EcoRI-ClaI fragment of human αN-catenin cDNA (haNctn) into the unique EcoRI and ClaI expression sites of pBluescriptIISK-.
When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide.
The nucleotide sequence of the haNctn cDNA corresponds with the EMBL Nucleotide Sequence Database (Release 35; Accession number M94151.2).
Other name of the plasmid is pαNcatEC.
EMBL Accession number: M94151.2, view at EMBL, GenBank, DDBJ
Latest sequence update: 11/04/1994
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr C. Petit(1).
(1) Institut Pasteur, Paris, France
Plasmid reference: Claverie et al., Genomics 15 (1993), 13-20 [PMID: 8432524]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pBluhANCTNf (LMBP 2911) is available at BCCM/GeneCorner. This plasmid was deposited by Dr C. Petit and was published in Claverie et al., 1993.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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