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GeneCorner plasmid details

Last data update: 26 October 2020 04:25 CET

Plasmid name: pBluhHemeOx (LMBP 2866)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p2866.gb (View with Genome Compiler)
p2866.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human heme oxygenase cDNA (hHemeOx, HMOX1)
Promoter: Phage T7 gene 10 promoter (T7g10)
Phage T3 promoter
Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Host range: Escherichia coli
Parental clone: pBluescriptIISK-
Further information: The plasmid was constructed by inserting the human heme oxygenase cDNA (hHemeOx) as an EcoRI fragment in the unique EcoRI site of the polylinker of pBluescriptIISK-.
The human heme oxygenase cDNA has an internal EcoRI site and can be isolated as two EcoRI fragments.
When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727551.1.
The nucleotide sequence of hHemeOx corresponds with the Genbank accession number X06985.1.
Name mentioned in Keyse et al. (1989) is clone 2/10.
Other name of the plasmid is pBlueSK-heme oxygen.
EMBL Accession number: X06985.1, view at EMBL, GenBank, DDBJ
LT727551.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 14/03/2006
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: ApaI, BanI, BglI and Eco47III.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr R.M. Tyrrell(1).
(1) University of Bath, United Kingdom
Plasmid reference: Keyse et al., Proc. Natl. Acad. Sci. U.S.A. 86 (1989), 99-103 [PMID: 2911585]
Related plasmid reference: Corrick et al., Gene 53 (1987), 63-71 [PMID: 3036667]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pBluhHemeOx (LMBP 2866) is available at BCCM/GeneCorner. This plasmid was deposited by Dr R.M. Tyrrell and was published in Keyse et al., 1989.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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