Last data update: 24 January 2024 16:39 CET
Plasmid name: pBode (LMBP 2597)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p2597.gb
(View with Genome Compiler) p2597.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
BK virus DNA (BKV); fragment of the Gardner strain |
| Promoter: | BK virus early promoter (BKV early) BK virus late promoter (BKV late) |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin BK virus origin (BKV) |
| Host range: | Escherichia coli Mammalian cells |
| Parental clone: | pML |
| Further information: | The plasmid was constructed by inserting the HindIII C-fragment of the BK virus (BKV; Gardner strain) into the unique HindIII site of pML (early region near to the EcoRI site of pML). pML (or pML-I) is a pBR322 derivative with deletion of the poison sequences (from pBR322 position 1094 to 2483). |
| EMBL Accession number: | - |
| Latest sequence update: | 03/11/2003 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI/EcoRI, EcoRI, HindIII and PvuII/XmnI. Referring to the expected fragment sizes deduced from the compiled nucleotide sequence, the presence, the length and the orientation of the BK virus insert could be confirmed, but the vector part of the plasmid seems to be 1000 bp larger. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr G. Barbanti-Brodano(1). (1) Institute of Microbiology, School of Medicine, University of Ferrara, Italy |
| Plasmid reference: | Milanesi et al., Mol. Cell. Biol. 4 (1984), 1551-1560 [PMID: 6092918] |
| Related plasmid reference: | Lusky et al., Nature 293 (1981), 79-81 [PMID: 6267479] Grossi et al., Arch. Virol. 99 (1988), 249-259 [PMID: 2835949] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pBode (LMBP 2597) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr G. Barbanti-Brodano and was published in Milanesi et al., 1984. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.