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GeneCorner plasmid details

Last data update: 23 October 2020 04:27 CEST

Plasmid name: pBode (LMBP 2597)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p2597.gb (View with Genome Compiler)
p2597.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: BK virus DNA (BKV); fragment of the Gardner strain
Promoter: BK virus early promoter (BKV early)
BK virus late promoter (BKV late)
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
BK virus origin (BKV)
Host range: Escherichia coli
Mammalian cells
Parental clone: pML
Further information: The plasmid was constructed by inserting the HindIII C-fragment of the BK virus (BKV; Gardner strain) into the unique HindIII site of pML (early region near to the EcoRI site of pML). pML (or pML-I) is a pBR322 derivative with deletion of the poison sequences (from pBR322 position 1094 to 2483).
EMBL Accession number: -
Latest sequence update: 03/11/2003
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BamHI/EcoRI, EcoRI, HindIII and PvuII/XmnI.

Referring to the expected fragment sizes deduced from the compiled nucleotide sequence, the presence, the length and the orientation of the BK virus insert could be confirmed, but the vector part of the plasmid seems to be 1000 bp larger.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr G. Barbanti-Brodano(1).
(1) Institute of Microbiology, School of Medicine, University of Ferrara, Italy
Plasmid reference: Milanesi et al., Mol. Cell. Biol. 4 (1984), 1551-1560 [PMID: 6092918]
Related plasmid reference: Grossi et al., Arch. Virol. 99 (1988), 249-259 [PMID: 2835949]
Lusky et al., Nature 293 (1981), 79-81 [PMID: 6267479]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pBode (LMBP 2597) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr G. Barbanti-Brodano and was published in Milanesi et al., 1984.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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