Last data update: 24 January 2024 16:39 CET
Plasmid name: pC2HB-Bem1 (LMBP 10473)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | - |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Candida albicans phosphatidylinositol-3-phosphate-binding protein cDNA (BEM1, GeneID 3635627) Staphylococcus aureus LexA repressor cDNA (LexA, lexA DNA-binding domain, lexA DBD, lexAbd, GeneID 3920198); N-terminal SV40 large T-antigen nuclear localization signal (NLS); N-terminal Influenza HA epitope encoding the haemagglutinin tagging peptide; N-terminal |
Promoter: | Candida albicans sulfate adenylyltransferase promoter (MET3) Phage SP6 promoter Phage T7 gene 10 promoter (T7g10) Escherichia coli lac operon promoter Escherichia coli β-lactamase promoter (amp) |
Ribosome binding site: |
- |
Terminator: | Candida albicans actin terminator (ACT1) |
Selection marker: | Neomycin (neo; kanamycin (kan)) Candida maltosa LEU2 (CmLEU2); auxotrophic |
Replicon: | Escherichia coli plasmid pMB1 origin |
Host range: | Escherichia coli Candida albicans; leu2(-), integrative |
Parental clone: | pC2HB-GC |
Further information: | The plasmid was constructed by cloning the C. albicans BEM1 coding sequence into the pC2HB-GC vector via Gateway LR recombination. This is a bait plasmid for yeast two-hybrid analysis. The plasmid can be linearised by NotI restriction to allow genomic integration. |
EMBL Accession number: | - |
Latest sequence update: | 15/04/2019 |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Prof. Dr P. Van Dijck(1) (2). It was constructed by F. Schoeters(1) (2). (1) VIB-KU Leuven Center for Microbiology, VIB, Leuven, Belgium (2) Laboratory of Molecular Cell Biology, Institute of Botany and Microbiology, KU Leuven, Leuven, Belgium |
Plasmid reference: | Schoeters et al., Front. Microbiol. 10 (2019), 1792 [PMID: 31440220] [DOI: 10.3389/fmicb.2019.01792] |
Related plasmid reference: | Schoeters et al., mSphere 3 (2018), e00391-18 [PMID: 30135223] [DOI: 10.1128/mSphere.00391-18] Legrand et al., Nucleic Acids Res. 46 (2018), 6935-6949 [PMID: 29982705] [DOI: 10.1093/nar/gky594] |
Restricted distribution: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 DH5α |
Host reference: | Focus 8 (1986), 9 |
Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
Cultivation medium: | LB-Lennox + kanamycin (50 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pC2HB-Bem1 (LMBP 10473) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr P. Van Dijck and was published in Schoeters et al., 2019. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.