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GeneCorner plasmid details

Last data update: 04 December 2020 04:17 CET

Plasmid name: pC2HB-GC (LMBP 9242)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p9242.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Candida albicans gDNA
B gene of the control of cell death locus of the Escherichia coli F plasmid (ccdB, lethal gene)
SV40 large T-antigen nuclear localization signal (NLS); N-terminal
Staphylococcus aureus LexA repressor cDNA (LexA, lexA DNA-binding domain, lexA DBD, lexAbd, GeneID 3920198); N-terminal
Influenza HA epitope encoding the haemagglutinin tagging peptide; N-terminal
Promoter: Candida albicans sulfate adenylyltransferase promoter (MET3)
Phage SP6 promoter
Phage T7 gene 10 promoter (T7g10)
Escherichia coli lac operon promoter
Escherichia coli β-lactamase promoter (amp)
Ribosome
binding site:
-
Terminator: Candida albicans actin terminator (ACT1)
Selection marker: Neomycin (neo; kanamycin (kan))
Candida maltosa LEU2 (CmLEU2); auxotrophic
Chloramphenicol (cam)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; use a ccdB-resistant strain for propagation
Candida albicans; leu2(-), integrative
Parental clone: pC2HB
Further information: The plasmid was created by cloning a Gateway cassette into the pC2HB vector.
This Gateway destination vector is meant as a bait plasmid for yeast two-hybrid analysis, in which the gene of interest can be cloned via Gateway recombination.
The plasmid can be linearised by NotI restriction to allow genomic integration.
This Gateway destination vector is compatible with the human orfeome library which is also available at BCCM/GeneCorner.
The nucleotide sequence of the S. aureus lexA cDNA corresponds with the EMBL Nucleotide Sequence Database accession number AY033082.1.
Other name of the plasmid is pC2HB-gc.
EMBL Accession number: AY033082.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 18/06/2014
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr P. Van Dijck(1) (2). It was constructed by Dr H. Tournu(1) (2) and Dr B. Stynen(1) (2).
(1) Department of Molecular Biology, VIB, Leuven, Belgium
(2) Laboratory of Molecular Cell Biology, Institute of Botany and Microbiology, KU Leuven, Leuven, Belgium
Plasmid reference: -
Related plasmid reference: Schoeters et al., mSphere 3 (2018) [PMID: 30135223] [DOI: 10.1128/mSphere.00391-18]
Restricted distribution: - BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12xB DB3.1
Host reference: -
Related host reference: Bernard et al., J. Mol. Biol. 226 (1992), 735-745 [PMID: 1324324]
Cultivation medium: LB-Lennox + chloramphenicol (34 μg/ml) + kanamycin (50 μg/ml)*
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: *: selection of transformants on chloramphenicol; subsequent cultivation of a single colony in liquid medium with kanamycin.
Other culture collection numbers: -

Refer in your Materials and Methods:

pC2HB-GC (LMBP 9242) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr P. Van Dijck .

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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