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GeneCorner plasmid details

Last data update: 29 October 2020 04:15 CET

Plasmid name: pC2HB-Pho85 (LMBP 10482)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p10482.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Candida albicans cyclin-dependent serine/threonine-protein kinase cDNA (PHO85, GeneID 3646148)
Staphylococcus aureus LexA repressor cDNA (LexA, lexA DNA-binding domain, lexA DBD, lexAbd, GeneID 3920198); N-terminal
SV40 large T-antigen nuclear localization signal (NLS); N-terminal
Influenza HA epitope encoding the haemagglutinin tagging peptide; N-terminal
Promoter: Candida albicans sulfate adenylyltransferase promoter (MET3)
Phage SP6 promoter
Phage T7 gene 10 promoter (T7g10)
Escherichia coli lac operon promoter
Escherichia coli β-lactamase promoter (amp)
Ribosome
binding site:
-
Terminator: Candida albicans actin terminator (ACT1)
Selection marker: Neomycin (neo; kanamycin (kan))
Candida maltosa LEU2 (CmLEU2); auxotrophic
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Candida albicans; leu2(-), integrative
Parental clone: pC2HB-GC
Further information: The plasmid was constructed by cloning the C. albicans PHO85 coding sequence into the pC2HB-GC vector via Gateway LR recombination.
This is a bait plasmid for yeast two-hybrid analysis.
The plasmid can be linearised by NotI restriction to allow genomic integration.
EMBL Accession number: -
Latest sequence update: 07/08/2018
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr F. Schoeters(1)(2) and Prof. Dr P. Van Dijck(1)(2). It was constructed by Dr H. Tournu(1)(2).
(1) VIB-KU Leuven Center for Microbiology, VIB, Leuven, Belgium
(2) Laboratory of Molecular Cell Biology, Institute of Botany and Microbiology, KU Leuven, Leuven, Belgium
Plasmid reference: Schoeters et al., mSphere 3 (2018) [PMID: 30135223] [DOI: 10.1128/mSphere.00391-18]
Related plasmid reference: Legrand et al., Nucleic Acids Res. (2018) [PMID: 29982705] [DOI: 10.1093/nar/gky594]
Schoeters et al., Front. Microbiol. 10 (2019), 1792 [PMID: 31440220] [DOI: 10.3389/fmicb.2019.01792]
Restricted distribution: - BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + kanamycin (50 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pC2HB-Pho85 (LMBP 10482) is available at BCCM/GeneCorner. This plasmid was deposited by Dr F. Schoeters and Prof. Dr P. Van Dijck and was published in Schoeters et al., 2018.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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