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GeneCorner plasmid details

Last data update: 27 November 2020 04:15 CET

Plasmid name: pC2HP-GC (LMBP 9244)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p9244.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Candida albicans gDNA
B gene of the control of cell death locus of the Escherichia coli F plasmid (ccdB, lethal gene)
SV40 large T-antigen nuclear localization signal (NLS); N-terminal
Viral protein 16 activation domain (VP16ad, AD VP16); N-terminal
FLAG epitope tag; N-terminal
Promoter: Candida albicans sulfate adenylyltransferase promoter (MET3)
Phage SP6 promoter
Escherichia coli lac operon promoter
Escherichia coli ß-lactamase promoter (amp)
Ribosome
binding site:
-
Terminator: Candida albicans actin terminator (ACT1)
Selection marker: Neomycin (neo; kanamycin (kan))
Candida dubliniensis ARG4 (CdARG4); auxotrophic
Chloramphenicol (cam)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; use a ccdB-resistant strain for propagation
Candida albicans; arg4(-), integrative
Parental clone: pC2HP
Further information: The plasmid was created by cloning a Gateway cassette into the pC2HP vector.
This Gateway destination vector is meant as a prey plasmid for yeast two-hybrid analysis, in which the protein partner of your gene of interest can be cloned via Gateway recombination.
The plasmid can be linearised by NotI restriction to allow genomic integration.
This Gateway destination vector is compatible with the human orfeome library which is also available at BCCM/GeneCorner.
Other name of the plasmid is pC2HP-gc.
EMBL Accession number: -
Latest sequence update: 30/04/2015
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr P. Van Dijck(1) (2). It was constructed by Dr B. Stynen(1) (2) and Dr H. Tournu(1) (2).
(1) Department of Molecular Biology, VIB, Leuven, Belgium
(2) Laboratory of Molecular Cell Biology, Institute of Botany and Microbiology, KU Leuven, Leuven, Belgium
Plasmid reference: -
Related plasmid reference: Schoeters et al., mSphere 3 (2018) [PMID: 30135223] [DOI: 10.1128/mSphere.00391-18]
Restricted distribution: - BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12xB DB3.1
Host reference: -
Related host reference: Bernard et al., J. Mol. Biol. 226 (1992), 735-745 [PMID: 1324324]
Cultivation medium: LB-Lennox + chloramphenicol (34 μg/ml) + kanamycin (50 μg/ml)*
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: *: selection of transformants on chloramphenicol; subsequent cultivation of a single colony in liquid medium with kanamycin.
Other culture collection numbers: -

Refer in your Materials and Methods:

pC2HP-GC (LMBP 9244) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr P. Van Dijck .

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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