Last data update: 20 October 2020 04:14 CEST
Plasmid name: pC2HP-Pbs2 (LMBP 10601)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Cloned DNA:||Candida albicans mitogen-activated protein kinase kinase cDNA (PBS2, GeneID 3641748)
Viral protein 16 activation domain (VP16ad, AD VP16); N-terminal
SV40 large T-antigen nuclear localization signal (NLS); N-terminal
FLAG epitope tag; N-terminal
|Promoter:||Candida albicans sulfate adenylyltransferase promoter (MET3)
Phage SP6 promoter
Escherichia coli lac operon promoter
Escherichia coli β-lactamase promoter (amp)
|Terminator:||Candida albicans actin terminator (ACT1)|
|Selection marker:||Neomycin (neo; kanamycin (kan))
Candida dubliniensis ARG4 (CdARG4); auxotrophic
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli
Candida albicans; arg4(-), integrative
|Further information:||The plasmid was constructed by cloning the C. albicans PBS2 coding sequence into the pC2HP-GC vector via Gateway LR recombination.
This is a prey plasmid for yeast two-hybrid analysis.
The plasmid can be linearised by NotI restriction to allow genomic integration.
|EMBL Accession number:||-|
|Latest sequence update:||16/04/2019|
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by Prof. Dr P. Van Dijck(1) (2). It was constructed by F. Schoeters(1) (2).
(1) VIB-KU Leuven Center for Microbiology, VIB, Leuven, Belgium
(2) Laboratory of Molecular Cell Biology, Institute of Botany and Microbiology, KU Leuven, Leuven, Belgium
|Plasmid reference:||Schoeters et al., Front. Microbiol. 10 (2019), 1792 [PMID: 31440220] [DOI: 10.3389/fmicb.2019.01792]
|Related plasmid reference:||Legrand et al., Nucleic Acids Res. (2018) [PMID: 29982705] [DOI: 10.1093/nar/gky594]
Schoeters et al., mSphere 3 (2018) [PMID: 30135223] [DOI: 10.1128/mSphere.00391-18]
|Restricted distribution:||- BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 MC1061|
|Host reference:||Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
|Related host reference:||Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
|Cultivation medium:||LB-Lennox + kanamycin (50 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pC2HP-Pbs2 (LMBP 10601) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr P. Van Dijck and was published in Schoeters et al., 2019.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.