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GeneCorner plasmid details

Last data update: 24 January 2021 04:18 CET

Plasmid name: pCA20 (LMBP 9316)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p9316.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Yersinia enterocolitica secretion protein P gene (yscP); modified sequence (yscP388)
Histidine tag (His-tag); N-terminal
Myc epitope; N-terminal
Promoter: Escherichia coli araBAD promoter (PBAD)
Ribosome
binding site:
Ribosome binding site (RBS) of the Escherichia coli L-ribulokinase gene (araB); optimized sequence
Terminator: Escherichia coli rrnB operon T1 terminator
Escherichia coli rrnB operon T2 terminator
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pLJ11
Further information: The plasmid was derived from pLJ11, deleting codons 46 to 96 (a spacer between two export signals) and 222 to 306 (one copy of the repeats in the central part) from yscP by inverse PCR and religating the fragments. This resulted in the Y. enterocolitica yscP388 CDS, cloned in the pBAD/Myc-HisA vector.
Other names of the plasmid are pBAD:yscP388+ and pBAD/Myc-HisA-yscP388.
EMBL Accession number: -
Latest sequence update: 03/07/2014
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr G. Cornelis(1). It was constructed by Dr C. Agrain(2).
(1) Research Unit in Microorganism Biology, University of Namur, Namur, Belgium
(2) Biozentrum, University of Basel, Switzerland
Plasmid reference: Journet et al., Science 302 (2003), 1757-1760 [PMID: 14657497]
Restricted distribution: - BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 LK111
Host reference: Zabeau et al., EMBO J. 1 (1982), 1217-1224 [PMID: 6327257]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pCA20 (LMBP 9316) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr G. Cornelis and was published in Journet et al., 2003.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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