Last data update: 24 January 2024 16:39 CET
Plasmid name: pCA20 (LMBP 9316)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p9316.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Yersinia enterocolitica secretion protein P gene (yscP); modified sequence (yscP388) Histidine tag (His-tag); N-terminal Myc epitope; N-terminal |
| Promoter: | Escherichia coli L-arabinose operon promoter (araB, araBAD, BAD) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli L-ribulokinase gene (araB); optimized sequence |
| Terminator: | Escherichia coli rrnB operon T1 terminator Escherichia coli rrnB operon T2 terminator |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pLJ11 |
| Further information: | The plasmid was derived from pLJ11, deleting codons 46 to 96 (a spacer between two export signals) and 222 to 306 (one copy of the repeats in the central part) from yscP by inverse PCR and religating the fragments. This resulted in the Y. enterocolitica yscP388 CDS, cloned in the pBAD/Myc-HisA vector. Other names of the plasmid are pBAD:yscP388+ and pBAD/Myc-HisA-yscP388. |
| EMBL Accession number: | - |
| Latest sequence update: | 03/07/2014 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr G. Cornelis(1). It was constructed by Dr C. Agrain(2). (1) Research Unit in Microorganism Biology, University of Namur, Namur, Belgium (2) Biozentrum, University of Basel, Switzerland |
| Plasmid reference: | Journet et al., Science 302 (2003), 1757-1760 [PMID: 14657497] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 LK111 |
| Host reference: | Zabeau et al., EMBO J. 1 (1982), 1217-1224 [PMID: 6327257] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pCA20 (LMBP 9316) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr G. Cornelis and was published in Journet et al., 2003. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.