GeneCorner plasmid details

Last data update: 01 July 2022 09:34 CEST

Plasmid name: pCAGGS (LMBP 2453)

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Price category: Cat. 3 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: (View with Genome Compiler)
analysis results

NGS: a04-gc-dec2018-partial.fasta

NGS: 2453-gc-jun2020.fasta

Cloned DNA: -
Promoter: Chicken β-actin/rabbit β-globin hybrid promoter (AG)
Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only
Escherichia coli lac operon promoter
binding site:
Terminator: Rabbit β-globin polyadenylation signal (β-globin polyA)
Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Parental clone: pUC13; pAGS-3
Further information: This plasmid is useful for highly efficient expression of genes under the control of the AG promoter and the human CMV-IE enhancer in various mammalian cells.
The AG promoter sequence consists of the chicken β-actin promoter, the first exon and part of the first intron (that seems to have a strong enhancer-like activity) linked to a rabbit β-globin fragment, consisting of a 3' part of the second intron (inclusive a branch point which is required for normal splicing reactions) and a 5' part of the third exon.
When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727518.1 except for the adjustments due to the implementation of the next-generation sequencing results.
EMBL Accession number: LT727518.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 05/11/2019
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BglII, EcoRI, NcoI, SacII and XhoI.

This plasmid has also been fully sequenced.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr J. Miyazaki(1).
(1) University of Tokyo, Japan
Plasmid reference: Niwa et al., Gene 108 (1991), 193-200 [PMID: 1660837]
Related plasmid reference: Lee et al., Mol. Cancer 10 (2011), 113 [PMID: 21917150] [DOI: 10.1186/1476-4598-10-113]
Thorrez et al., Mol. Ther. 14 (2006), 442-451 [PMID: 16750937] [DOI: 10.1016/j.ymthe.2006.03.019]
Verhelst et al., EMBO J. 31 (2012), 3845-3855 [PMID: 23032186] [DOI: 10.1038/emboj.2012.240]
Staal et al., bioRxiv (2017) [DOI: 10.1101/228767]
Pattabriaman et al., J. Cell Biol. 208 (2015), 53-69 [PMID: 25547156] [DOI: 10.1083/jcb.201404109]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pCAGGS (LMBP 2453) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr J. Miyazaki and was published in Niwa et al., 1991.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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