Last data update: 24 January 2024 16:39 CET
Plasmid name: pCAGGS-E-CFP-A20 (LMBP 6025)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p6025.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human TNF alpha induced protein 3 cDNA (TNFAIP3, A20, OTUD7C, GeneID 7128) E-tag; N-terminal Aequorea victoria green fluorescent protein DNA (GFP); enhanced cyan fluorescent variant (ECFP), N-terminal |
| Promoter: | Chicken β-actin/rabbit β-globin hybrid promoter (AG) Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Rabbit β-globin polyadenylation signal (β-globin polyA) Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pCAGGSEhA20; pdECFP |
| Further information: | The plasmid was constructed by PCR amplifying the human TNFAIP3 coding sequence from pCAGGSEhA20 and the ECFP coding sequence from pdECFP. Both coding sequences were fused and ligated as a NotI/NsiI fragment into the pCAGGSEhA20 vector. The nucleotide sequence of the human TNFAIP3 cDNA corresponds with the EMBL Nucleotide Sequence Database accession number M59465.1. |
| EMBL Accession number: | M59465.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 10/03/2009 |
| Sequence detail: | Primers used for creating the ECFP-hA20 fusion product:
A20-F : ATG.GCT.GAA.CAA.GTC.CTT.CC
***
NotI-CFP-F : CCGCCTGCGGCCGCA.ATG.GTG.AGC.AAG.GGC.GAG.GAG.C
NotI ###
CFP-A20-R : GG.AAG.GAC.TTG.TTC.AGC.CAT.CTT.GTA.CAG.CTC.GTC.CAT.GCC.G
***
***: start codon of hA20 cds.
###: start codon of eCFP cds. |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr J. Staal(1) (2) and Prof. Dr R. Beyaert(1) (2). (1) UGent-VIB Center for Inflammation Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pCAGGS-E-CFP-A20 (LMBP 6025) is available at BCCM/GeneCorner. This plasmid was deposited by Dr J. Staal and Prof. Dr R. Beyaert . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.