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GeneCorner plasmid details

Last data update: 31 October 2020 04:21 CET

Plasmid name: pCAGGS-E-CgBcl10 (LMBP 9666)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p9666.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Crassostrea gigas B-cell lymphoma/leukemia 10-like cDNA (Bcl10, GeneID 105331345)
E-tag; N-terminal
Promoter: Chicken β-actin/rabbit β-globin hybrid promoter (AG)
Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only
Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: Rabbit β-globin polyadenylation signal (β-globin polyA)
Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Parental clone: pCAGGSEhA20
Further information: The plasmid was constructed by PCR amplifying the C. gigas Bcl10 coding sequence from a cDNA library, and cloning it into the NotI/XhoI opened pCAGGSEhA20 vector, replacing the human TNFAIP3 coding sequence.
EMBL Accession number: -
Latest sequence update: 31/10/2018
Sequence detail:
Primers used to amplify the C. gigas BCL10 coding sequence:

Forward: 5' ACCGCGTGCGGCCGCA.ATG.AAT.GGG.AAT.AAA.ATA.AAA.GAG.GA
                   NotI      ***

Reverse: 5' TCGACCTCGAG.AGC.GTT.CGA.ATC.ATT.GCT.AGA.G
                 XhoI


***: Starting codon
Punctuation indicates reading frame.
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr J. Staal(1) (2) and Prof. Dr R. Beyaert(1) (2).
(1) UGent-VIB Center for Inflammation Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Staal et al. (2016) [DOI: dx.doi.org/10.1101/046789]
Related plasmid reference: Staal et al., Front Immunol 9 (2018), 1136 [PMID: 29881386] [DOI: 10.3389/fimmu.2018.01136]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pCAGGS-E-CgBcl10 (LMBP 9666) is available at BCCM/GeneCorner. This plasmid was deposited by Dr J. Staal and Prof. Dr R. Beyaert and was published in Staal et al., 2016.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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