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GeneCorner plasmid details

Last data update: 28 October 2020 04:17 CET

Plasmid name: pCAGGS-E-NvBcl10 (LMBP 9822)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p9822.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Nematostella vectensis B cell CLL/lymphoma 10 homolog cDNA (Bcl10)
E-tag; C-terminal
Promoter: Chicken β-actin/rabbit β-globin hybrid promoter (AG)
Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only
Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: Rabbit β-globin polyadenylation signal (β-globin polyA)
Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Parental clone: pCAGGSEhA20; pUC19-BcABA4-TwinStrep-NvBcl10
Further information: The plasmid was constructed by isolating the Nematostella BCL10 coding sequence from pUC19-BcABA4-TwinStrep-NvBcl10, and cloning it into the NotI/XhoI opened pCAGGSEhA20 vector, replacing the human A20 coding sequence.
Bcl10 in cnidaria, such as Nematostella, are the most distantly related Bcl10 homologs currently known.
EMBL Accession number: -
Latest sequence update: 01/10/2018
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BglI, BglII, EcoRV/HincII, NcoI and NotI/XhoI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr J. Staal(1) (2) and Prof. Dr R. Beyaert(1) (2).
(1) Center for Inflammation Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Staal et al., bioRxiv (2016) [DOI: 10.1101/046789]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pCAGGS-E-NvBcl10 (LMBP 9822) is available at BCCM/GeneCorner. This plasmid was deposited by Dr J. Staal and Prof. Dr R. Beyaert and was published in Staal et al., 2016.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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