Last data update: 24 January 2024 16:39 CET
Plasmid name: pCAGGS-E-OPTNsm (LMBP 7618)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p7618.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human optineurin cDNA (OPTN, FIP2, GLC1E, HYPL, HIP7, GeneID 10133); mutated sequence E-tag; N-terminal |
| Promoter: | Chicken β-actin/rabbit β-globin hybrid promoter (AG) Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Rabbit β-globin polyadenylation signal (β-globin polyA) Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pCAGGS |
| Further information: | The plasmid contains the full length human OPTN coding sequence with silent mutations. When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide. The nucleotide sequence of the wild type human OPTN cDNA corresponds with the Genbank accession number NM_001008213.1. Other name of the plasmid is pCAGGS-E-OPTN-FL*. |
| EMBL Accession number: | NM_001008213.1, view at GenBank |
| Latest sequence update: | 02/04/2012 |
| Sequence detail: | Silent mutations in the human OPTN coding sequence: Wild type: TCC TCA GAA GAT TCC TTT Mutation: AGT AGC GAG GAC AGT TTT |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BstXI/EcoRI, HincII/NcoI, HindIII and NotI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by J. Staal(1) (2) and Prof. Dr R. Beyaert(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pCAGGS-E-OPTNsm (LMBP 7618) is available at BCCM/GeneCorner. This plasmid was deposited by J. Staal and Prof. Dr R. Beyaert . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.