Last data update: 24 January 2024 16:39 CET
Plasmid name: pCAGGS-E-RIG-I-CARD (LMBP 6517)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p6517.gb
(View with Genome Compiler) p6517.txt p6517.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human DExD/H-box helicase 58 cDNA (DDX58, RIG-I); fragment E-tag; N-terminal |
| Promoter: | Chicken β-actin/rabbit β-globin hybrid promoter (AG) Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Rabbit β-globin polyadenylation signal (β-globin polyA) Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pCAGGSEhA20; pMET7-RIG-I-CARD |
| Further information: | The plasmid was constructed by PCR amplifying the caspase recruitment domain (CARD) of human DDX58 from pMET7-RIG-I-CARD and cloning it into the XhoI/ NotI opened pCAGGSEhA20 plasmid, replacing the human A20 cds. The plasmid is useful for highly efficient expression of the E-tagged human DDX58 CARD domain under the control of the AG promoter and the human CMV-IE enhancer in various mammalian cells. When cloning a fragment downstream from the lac promoter it may be advisable to use lacIq strains in order to prevent fortuitous expression of a possibly noxious polypeptide. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT726833.1. The nucleotide sequence of the human DDX58 CARD domain corresponds with the EMBL Nucleotide Sequence Database accession number AF038963.1. |
| EMBL Accession number: | AF038963.1, view at EMBL, GenBank, DDBJ LT726833.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 03/12/2009 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BglII/NcoI, HindIII and NotI/XhoI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr R. Beyaert(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Related plasmid reference: | Maelfait et al., PLoS Pathog. 8 (2012), e1002570 [PMID: 22396652] [DOI: 10.1371/journal.ppat.1002570] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pCAGGS-E-RIG-I-CARD (LMBP 6517) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Beyaert . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.