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GeneCorner plasmid details

Last data update: 03 December 2020 04:26 CET

Plasmid name: pCAGGS-E-RIG-I-CARD (LMBP 6517)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p6517.gb (View with Genome Compiler)
p6517.txt
p6517.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human DExD/H-box helicase 58 cDNA (DDX58, RIG-I); fragment
E-tag; N-terminal
Promoter: Chicken β-actin/rabbit β-globin hybrid promoter (AG)
Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only
Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: Rabbit β-globin polyadenylation signal (β-globin polyA)
Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Parental clone: pCAGGSEhA20; pMET7-RIG-I-CARD
Further information: The plasmid was constructed by PCR amplifying the caspase recruitment domain (CARD) of human DDX58 from pMET7-RIG-I-CARD and cloning it into the XhoI/ NotI opened pCAGGSEhA20 plasmid, replacing the human A20 cds.
The plasmid is useful for highly efficient expression of the E-tagged human DDX58 CARD domain under the control of the AG promoter and the human CMV-IE enhancer in various mammalian cells.
When cloning a fragment downstream from the lac promoter it may be advisable to use lacIq strains in order to prevent fortuitous expression of a possibly noxious polypeptide.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT726833.1.
The nucleotide sequence of the human DDX58 CARD domain corresponds with the EMBL Nucleotide Sequence Database accession number AF038963.1.
EMBL Accession number: AF038963.1, view at EMBL, GenBank, DDBJ
LT726833.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 03/12/2009
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BglII/NcoI, HindIII and NotI/XhoI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr R. Beyaert(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Related plasmid reference: Maelfait et al., PLoS Pathog. 8 (2012), e1002570 [PMID: 22396652] [DOI: 10.1371/journal.ppat.1002570]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pCAGGS-E-RIG-I-CARD (LMBP 6517) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Beyaert .

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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