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GeneCorner plasmid details

Last data update: 29 October 2020 04:15 CET

Plasmid name: pCAGGS-E-hA20-C103S (LMBP 5355)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p5355.gb (View with Genome Compiler)
p5355.txt
p5355.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human TNF alpha induced protein 3 cDNA (TNFAIP3, A20, OTUD7C, GeneID 7128); mutated sequence
E-tag; N-terminal
Promoter: Chicken β-actin/rabbit β-globin hybrid promoter (AG)
Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only
Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: Rabbit β-globin polyadenylation signal (β-globin polyA)
Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Parental clone: pAS2-hA20-C103S; pCAGGSEhA20
Further information: The plasmid was constructed by inserting the EcoNI-HpaI fragment from pAS2-hA20-C103S into the EcoNI-HpaI opened pCAGGSEhA20 vector.
pCAGGS-E-hA20-C103S is useful for efficient expression of mutated hA20 under the control of the AG promoter and the hCMV-IE enhancer in various mammalian cells.
The hA20 insert contains a mutation in the ovarian tumor (OTU) domain (C103S).
When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727012.1.
The nucleotide sequence of the wt hA20 cDNA corresponds with the EMBL Nucleotide Sequence Database accession number M59465.1.
Other name of the plasmid is pCAGGS-E-A20(C103S).
EMBL Accession number: M59465.1, view at EMBL, GenBank, DDBJ
LT727012.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 02/02/2009
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: Bsu36I, HindIII, XhoI and XmnI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr K. Heyninck(1) (2) and Prof. Dr R. Beyaert(1) (2). It was constructed by M. Kreike(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pCAGGS-E-hA20-C103S (LMBP 5355) is available at BCCM/GeneCorner. This plasmid was deposited by Dr K. Heyninck and Prof. Dr R. Beyaert .

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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