Last data update: 24 January 2024 16:39 CET
Plasmid name: pCAGGS-E-hA20R439K (LMBP 6085)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p6085.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human TNF alpha induced protein 3 cDNA (TNFAIP3, A20, OTUD7C, GeneID 7128); mutated sequence E-tag; N-terminal |
| Promoter: | Chicken β-actin/rabbit β-globin hybrid promoter (AG) Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Rabbit β-globin polyadenylation signal (β-globin polyA) Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pCAGGSEhA20 |
| Further information: | The hA20 mutated sequence was PCR amplified and inserted as an HpaI-SacI fragment into the HpaI-SacI opened pCAGGSEhA20 vector. The hA20 coding sequence contains an R439K mutation. |
| EMBL Accession number: | M59465, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 17/02/2009 |
| Sequence detail: | R439K mutagenesis primers:
435 439 440 445
A20R/K-F: ATG.GCG.CTC.GGG.GCC.TCT.AAG.GGA.GAA.GCC.TAT.GAG.CC
^
445 440 439 435
A20R/K-R : GG.CTC.ATA.GGC.TTC.TCC.CTT.AGA.GGC.CCC.GAG.CGC.CAT
^
270 274
F(HpaI) : c.cga.gct.gtt.cca.ctt.gtt.aac.aga.gac
HpaI
735 730
R (SacI) : atg.ctg.aca.ctc.cat.gca.gag.ctc.c
SacI
^: mutated nucleotide |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr R. Beyaert(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Related plasmid reference: | Hulpiau et al., Cell. Mol. Life Sci. 73 (2016), 1103-1116 [PMID: 26377317] [DOI: 10.1007/s00018-015-2041-9] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pCAGGS-E-hA20R439K (LMBP 6085) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Beyaert . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.