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GeneCorner plasmid details

Last data update: 03 December 2020 04:26 CET

Plasmid name: pCAGGS-E-hA20R439K (LMBP 6085)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p6085.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human TNF alpha induced protein 3 cDNA (TNFAIP3, A20, OTUD7C, GeneID 7128); mutated sequence
E-tag; N-terminal
Promoter: Chicken β-actin/rabbit β-globin hybrid promoter (AG)
Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only
Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: Rabbit β-globin polyadenylation signal (β-globin polyA)
Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Parental clone: pCAGGSEhA20
Further information: The hA20 mutated sequence was PCR amplified and inserted as an HpaI-SacI fragment into the HpaI-SacI opened pCAGGSEhA20 vector.
The hA20 coding sequence contains an R439K mutation.
EMBL Accession number: M59465, view at EMBL, GenBank, DDBJ
Latest sequence update: 17/02/2009
Sequence detail:
R439K mutagenesis primers:

                  435             439 440                 445 
A20R/K-F: ATG.GCG.CTC.GGG.GCC.TCT.AAG.GGA.GAA.GCC.TAT.GAG.CC
                                   ^ 
          445                 440 439             435
A20R/K-R : GG.CTC.ATA.GGC.TTC.TCC.CTT.AGA.GGC.CCC.GAG.CGC.CAT
                                   ^
                270             274 
F(HpaI) : c.cga.gct.gtt.cca.ctt.gtt.aac.aga.gac
                                  HpaI

               735                 730
R (SacI) : atg.ctg.aca.ctc.cat.gca.gag.ctc.c
                                     SacI

^: mutated nucleotide
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr R. Beyaert(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Related plasmid reference: Hulpiau et al., Cell. Mol. Life Sci. 73 (2016), 1103-1116 [PMID: 26377317] [DOI: 10.1007/s00018-015-2041-9]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pCAGGS-E-hA20R439K (LMBP 6085) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Beyaert .

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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