Last data update: 28 November 2020 04:24 CET
Plasmid name: pCAGGS-E-hABIN-1-MAD (LMBP 5131)
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p5131.gb
(View with Genome Compiler) p5131.txt p5131.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: | Human A20-binding inhibitor of NF-κB activation 1 cDNA (ABIN-1, TNIP1, NAF1); minimal active domain (MAD) E-tag; N-terminal |
| Promoter: | Chicken β-actin/rabbit β-globin hybrid promoter (AG) Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Rabbit β-globin polyadenylation signal (β-globin polyA) Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pCAGGS-E-hABIN-1-P299A |
| Further information: | The plasmid was constructed by inserting a 485 bp NotI-XhoI PCR fragment, containing the minimal active domain (codons 431 up to and including 588) of the human ABIN-1 coding sequence, into the NotI-XhoI opened pCAGGS-E-hABIN-1-P299A vector. pCAGGS-E-hABIN-1-MAD is useful for highly efficient expression of human ABIN-1-MAD, fused in phase to the N-terminal E-tag, under the control of the AG promoter and the human CMV-IE enhancer in various mammalian cells. When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727095.1. The nucleotide sequence of the human ABIN-1-MAD sequence corresponds with the EMBL Nucleotide Sequence Database accession number AJ011895.1. Other name of the plasmid is pCAGGS-E-hABIN1-MAD. |
| EMBL Accession number: | AJ011895.1, view at EMBL, GenBank, DDBJ LT727095.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 03/01/2006 |
| Sequence detail: | Primers used to amplify the minimal active domain of the human ABIN-1 coding sequence:
431 435
forward: 5' ... AAGGAAAAAAGCGGCCGCC.ACC.CCG.CCA.TCA.TCT.CCA.CC ... 3'
NotI
588 585
reverse: 5' ... GCCGCTCGAG.TCA.CGA.GTT.GGG.GAG.TGG.GGG ... 3'
XhoI +++
+++: termination codon.
Punctuation indicates reading frame. |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: ApaI, HincII, NotI/XhoI and SacII/XmnI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr R. Beyaert(1) (2). It was constructed by M. Haegman(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Related plasmid reference: | Huang et al., Oncogene 27 (2008), 6131-6140 [PMID: 18622428] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
|
Refer in your Materials and Methods: |
pCAGGS-E-hABIN-1-MAD (LMBP 5131) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Beyaert . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.