Last data update: 24 January 2024 16:39 CET
Plasmid name: pCAGGS-E-hCCR3 (LMBP 7782)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p7782.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human chemokine (C-C motif) receptor 3 (CCR3) E-tag; N-terminal |
| Promoter: | Chicken β-actin/rabbit β-globin hybrid promoter (AG) Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Rabbit β-globin polyadenylation signal (β-globin polyA) Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pCDM8-hCCR3; pCAGGS-E-CYLD |
| Further information: | The plasmid was created by isolating the human CCR3 CDS from pCDM8-hCCR3 by PCR and cloning it into the NotI/XhoI opened pCAGGS-E-CYLD vector, thereby replacing the CYLD CDS. The nucleotide sequence of the human CCR3 cDNA corresponds with the EMBL Nucleotide Sequence Database accession number AF247361.1. |
| EMBL Accession number: | AF247361.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 19/09/2012 |
| Sequence detail: | Primers used to isolate the human CCR3 CDS:
Forward: 5' TCGTGCGGCCGCA.ATG.ACA.ACC.TCA.CTA.GAT.ACA.GTT.G
NotI ***
Reverse: 5' AGGCCTCGAG.CTA.AAA.CAC.AAT.AGA.GAG.TTC.CGG.C
XhoI +++
***: start codon.
+++: termination codon.
Punctuation indicates reading frame. |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr J. Staal(1) (2) and Prof. Dr R. Beyaert(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pCAGGS-E-hCCR3 (LMBP 7782) is available at BCCM/GeneCorner. This plasmid was deposited by Dr J. Staal and Prof. Dr R. Beyaert . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.