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GeneCorner plasmid details

Last data update: 30 October 2020 04:11 CET

Plasmid name: pCAGGS-E-hCCR3 (LMBP 7782)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p7782.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human chemokine (C-C motif) receptor 3 (CCR3)
E-tag; N-terminal
Promoter: Chicken β-actin/rabbit β-globin hybrid promoter (AG)
Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only
Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: Rabbit β-globin polyadenylation signal (β-globin polyA)
Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Parental clone: pCDM8-hCCR3; pCAGGS-E-CYLD
Further information: The plasmid was created by isolating the human CCR3 CDS from pCDM8-hCCR3 by PCR and cloning it into the NotI/XhoI opened pCAGGS-E-CYLD vector, thereby replacing the CYLD CDS.
The nucleotide sequence of the human CCR3 cDNA corresponds with the EMBL Nucleotide Sequence Database accession number AF247361.1.
EMBL Accession number: AF247361.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 19/09/2012
Sequence detail:
Primers used to isolate the human CCR3 CDS:

Forward: 5' TCGTGCGGCCGCA.ATG.ACA.ACC.TCA.CTA.GAT.ACA.GTT.G 
                NotI      ***

Reverse: 5' AGGCCTCGAG.CTA.AAA.CAC.AAT.AGA.GAG.TTC.CGG.C
                XhoI   +++

***: start codon.
+++: termination codon.
Punctuation indicates reading frame.
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr J. Staal(1) (2) and Prof. Dr R. Beyaert(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pCAGGS-E-hCCR3 (LMBP 7782) is available at BCCM/GeneCorner. This plasmid was deposited by Dr J. Staal and Prof. Dr R. Beyaert .

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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