Last data update: 27 November 2020 04:15 CET
Plasmid name: pCAGGS-E-hPELI2-DN (LMBP 5194)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Cloned DNA:||Human pellino 2 cDNA (PELI2); C-terminal part encoding amino acids 328-420, RING-domain only
|Promoter:||Chicken β-actin/rabbit β-globin hybrid promoter (AG)
Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only
Escherichia coli lac operon promoter
|Terminator:||Rabbit β-globin polyadenylation signal (β-globin polyA)
Simian virus 40 polyadenylation signal (SV40 polyA)
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin
Simian virus 40 bidirectional origin (SV40)
|Host range:||Escherichia coli
Mammalian cells; SV40 permissive cells
|Further information:||The plasmid was constructed as follows: 1) a splice variant of the human PELI2 cDNA, encoding amino acids 328 up to 420, was generated by PCR; 2) 2) the NotI/XhoI digested PCR amplicon was fused in frame to the E-tag of a NotI/XhoI opened pCAGGS-based expression plasmid.
The nucleotide sequence of the hPELI2 cDNA corresponds with the EMBL Nucleotide Sequence Database accession number AF302502.1.
Other name of the plasmid is pCAGGS-E-hPeli-2DN.
|EMBL Accession number:||AF302502.1, view at EMBL, GenBank, DDBJ|
|Latest sequence update:||14/09/2007|
The primers used in PCR to amplify hPELI2-DN cDNA were: forward: 5' ATAAGAATGCGGCCGCAACGGAGGCCAACGAGAGGGA 3' NotI reverse : 5' CCGCTCGAGTCAGTCAATTGGACCTTGGAAA 3' XhoI
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by Prof. Dr R. Beyaert(1) (2). It was constructed by Dr R. Schauvliege(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Related plasmid reference:||Schauvliege et al., FEBS Lett. 580 (2006), 4697-4702 [PMID: 16884718]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 MC1061|
|Host reference:||Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
|Related host reference:||Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pCAGGS-E-hPELI2-DN (LMBP 5194) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Beyaert .|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.