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GeneCorner plasmid details

Last data update: 23 October 2020 04:27 CEST

Plasmid name: pCAGGS-E-hsiF (LMBP 6929)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p6929.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Pseudomonas aeruginosa PAO1 hsiF cDNA
E-tag; N-terminal
Promoter: Chicken β-actin/rabbit β-globin hybrid promoter (AG)
Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only
Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: Rabbit β-globin polyadenylation signal (β-globin polyA)
Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Parental clone: pCAGGSEhA20
Further information: The plasmid was constructed by replacing the hA20 cds from the NotI/BglII opened pCAGGSEhA20 by the PCR amplified hsiF1 cds.
Protein expression of this vector in HEK293T cells was tested at GeneCorner.
EMBL Accession number: NC_002516, view at EMBL, GenBank, DDBJ
Latest sequence update: 01/02/2010
Sequence detail:
AAGGAAGCGGCCGCTgccgaactgaccctccag
GTCGCCTCGAGtcatgtacgcctccgctc
Primers used to isolate the HsiF cDNA:

Forward primer: 5' AAGGAAGCGGCCGCT.GCC.GAA.CTG.ACC.CTC.CAG
                         NotI      AA2  
Reverse primer: 5' GTCGCCTCGAG.TCA.TGT.AG.CT.CCG.CTC
                        XhoI   +++ 

+++: termination codon
Punctuation indicates reading frame.
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by W. De Schamphelaire(1) and Prof. Dr R. Beyaert(1).
(1) BCCM/GeneCorner, Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pCAGGS-E-hsiF (LMBP 6929) is available at BCCM/GeneCorner. This plasmid was deposited by W. De Schamphelaire and Prof. Dr R. Beyaert.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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