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GeneCorner plasmid details

Last data update: 02 December 2020 04:29 CET

Plasmid name: pCAGGS-E-mCYLD (LMBP 6692)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p6692.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Mouse CYLD lysine 63 deubiquitinase cDNA (Cyld, CYLD1)
E-tag; N-terminal
Promoter: Chicken β-actin/rabbit β-globin hybrid promoter (AG)
Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only
Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: Rabbit β-globin polyadenylation signal (β-globin polyA)
Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Parental clone: pCD-F-mCYLD; pCAGGS-E
Further information: The plasmid was constructed by PCR amplifying the mCyld CDS and ligating it as an NotI/XhoI fragment into the NotI/XhoI opened pCAGGS-E vector. The clone was confirmed to be expressed in HEK293T cells.
EMBL Accession number: -
Latest sequence update: 19/04/2011
Sequence detail:
Primers used to amplify the mCyld CDS:

Forward: 5' CTCATGCGGCCGCA.ATG.AGT.TCA.GGC.CTG.TGG.A 
                 NotI      ***

Reverse: 5' ATAACTCGAGGTC.TTA.TTT.GTA.CAG.GCT.CAT.GGT.TGG
                XhoI      +++

***: start codon.
+++: termination codon.
Punctuation indicates reading frame.
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr J. Staal(1) (2) and Prof. Dr R. Beyaert(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Staal et al., EMBO J. 30 (2011), 1742-1752 [PMID: 21448133] [DOI: 10.1038/emboj.2011.85]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pCAGGS-E-mCYLD (LMBP 6692) is available at BCCM/GeneCorner. This plasmid was deposited by Dr J. Staal and Prof. Dr R. Beyaert and was published in Staal et al., 2011.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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