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GeneCorner plasmid details

Last data update: 23 October 2020 04:27 CEST

Plasmid name: pCAGGS-E-mMyD88-L265P (LMBP 9635)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p9635.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Mouse myeloid differentiation primary response gene 88 cDNA (Myd88, GeneID 17874); mutated sequence
E-tag; N-terminal
Promoter: Chicken β-actin/rabbit β-globin hybrid promoter (AG)
Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only
Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: Rabbit β-globin polyadenylation signal (β-globin polyA)
Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Parental clone: pCAGGS-E-mMyD88
Further information: The plasmid was created by introducing an L265P mutation in the mouse MyD88 coding sequence of pCAGGS-E-mMyD88.
The L265P somatic mutation has been found in multiple B-cell lymphomas (Ngo et al., 2011 and Montesinos-Rongen et al., 2011). Interestingly, the same B-cell lymphomas that were having this mutation are also responsive to MALT1 inhibitor treatments (Ferch et al., 2009 and Hailfinger et al., 2009). This clone was made in order to see whether the mutated MyD88 activates a MALT1-dependent pathway. No effect was observed on MALT1 activity.
Other name of the plasmid is pCAGGS-E-MyD88-L265P.
EMBL Accession number: -
Latest sequence update: 07/01/2016
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr J. Staal(1) (2) and Prof. Dr R. Beyaert(1) (2).
(1) Inflammation Research Center, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Related plasmid reference: Ngo et al., Nature 470 (2011), 115-119 [PMID: 21179087] [DOI: 10.1038/nature09671]
Montesinos-Rongen et al., Acta Neuropathol. 122 (2011), 791-792 [PMID: 22020631] [DOI: 10.1007/s00401-011-0891-2]
Ferch et al., J. Exp. Med. 206 (2009), 2313-2320 [PMID: 19841089] [DOI: 10.1084/jem.20091167]
Hailfinger et al., Proc. Natl. Acad. Sci. U.S.A. 106 (2009), 19946-19951 [PMID: 19897720] [DOI: 10.1073/pnas.0907511106]
Zhang et al., Nature 481 (2011), 204-208 [PMID: 22158122] [DOI: 10.1038/nature10690]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pCAGGS-E-mMyD88-L265P (LMBP 9635) is available at BCCM/GeneCorner. This plasmid was deposited by Dr J. Staal and Prof. Dr R. Beyaert .

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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