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GeneCorner plasmid details

Last data update: 20 October 2020 04:14 CEST

Plasmid name: pCAGGS-E-mMyD88-TIR (LMBP 5647)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p5647.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Mouse myeloid differentiation primary response gene 88 cDNA (Myd88, GeneID 17874); C-terminal fragment (Myd88-TIR)
E-tag; N-terminal
Promoter: Chicken β-actin/rabbit β-globin hybrid promoter (AG)
Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only
Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: Rabbit β-globin polyadenylation signal (β-globin polyA)
Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Parental clone: pCAGGS-E-mMyD88
Further information: The plasmid was constructed by PCR amplifying the C-terminal fragment of mMyD88 (codons 155-296) and ligating it as a NotI/XmaI fragment into pCAGGS-E.
This plasmid is useful for highly efficient expression of mMyD88-TIR under the control of the AG promoter and the human CMV-IE enhancer in various mammalian cells.
When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide.
The nucleotide sequence of the mouse Myd88 cDNA corresponds with the EMBL Nucleotide Sequence Database accession number U84409.1.
EMBL Accession number: U84409.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 23/01/2008
Sequence detail:
Primers used to amplify the mMyD88-TIR cds: 
                                             |155 156 157 158
Forward primer: 5' TA.GAA.TTC.GCG.GCC.GCG.ATG.GGA.CAA.ACG.CCG.G 3'
                      EcoRI   NotI        ***

                                  296 295 294 293 292
Reverse primer: 5' CGCCCCCGGG.TCA.GGG.CAG.GGA.CAA.AGC 3'
                       XmaI   +++

***: start codon.
+++: termination codon.
Punctuation indicates reading frame.
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr S. Janssens(1) (2) and Prof. Dr R. Beyaert(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Related plasmid reference: Janssens et al., Curr. Biol. 12 (2002), 467-471 [PMID: 11909531]
Janssens et al., Trends Biochem. Sci. 27 (2002), 474-482 [PMID: 12217523]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pCAGGS-E-mMyD88-TIR (LMBP 5647) is available at BCCM/GeneCorner. This plasmid was deposited by Dr S. Janssens and Prof. Dr R. Beyaert .

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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