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GeneCorner plasmid details

Last data update: 26 November 2020 04:30 CET

Plasmid name: pCAGGS-E-mXedar-E232K-E256K (LMBP 6060)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p6060.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Mouse ectodysplasin A2 receptor (Xedar, Eda2r); mutated sequence
E-tag; N-terminal
Promoter: Chicken β-actin/rabbit β-globin hybrid promoter (AG)
Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only
Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: Rabbit β-globin polyadenylation signal (β-globin polyA)
Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Parental clone: pCAGGS-E-mXedar-E232K
Further information: The plasmid was created by inserting a E256K mutation in the mouse Xedar-E232K mutant coding sequence from pCAGGS-E-mXedar-E232K via PCR mutagenesis. The double mutated mouse Xedar coding sequence was isolated and used to replace the single mutant coding sequence in pCAGGS-E-mXedar-E232K as an XhoI-MfeI fragment.
Other name of the plasmid is pCAGGS-E-mXEDAR E232K/E256K.
EMBL Accession number: -
Latest sequence update: 10/04/2009
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr K. Verhelst(1) (2) and Prof. Dr R. Beyaert(1) (2).
(1) Inflammation Research Center, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pCAGGS-E-mXedar-E232K-E256K (LMBP 6060) is available at BCCM/GeneCorner. This plasmid was deposited by Dr K. Verhelst and Prof. Dr R. Beyaert .

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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