Last data update: 24 January 2024 16:39 CET
Plasmid name: pCAGGS-E-pipB (LMBP 6731)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | p6731.gb |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Salmonella enterica serovar Typhimurium LT2 pathogenicity-island encoded protein B cDNA (pipB) E-tag; N-terminal |
Promoter: | Chicken β-actin/rabbit β-globin hybrid promoter (AG) Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only Escherichia coli lac operon promoter |
Ribosome binding site: |
- |
Terminator: | Rabbit β-globin polyadenylation signal (β-globin polyA) Simian virus 40 polyadenylation signal (SV40 polyA) |
Selection marker: | Ampicillin (amp) |
Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
Parental clone: | pCAGGSEhA20 |
Further information: | The plasmid was constructed by replacing the hA20 cds from the NotI/XhoI opened pCAGGSEhA20 by the PCR amplified pipB cds. Protein expression of this vector in HEK293T cells was tested at GeneCorner. |
EMBL Accession number: | AE006468, view at EMBL, GenBank, DDBJ |
Latest sequence update: | 06/08/2010 |
Sequence detail: | Primers used to isolate the PipB cDNA: Forward primer: 5' AAGGAAGCGGCCGCT.CCA.ATA.ACT.AAC.GCG.TCC.C NotI AA2 Reverse primer: 5' GTCGCCTCGAG.CTA.AAA.TAT.CGG.ATG.GGG.GAA XhoI +++ +++: termination codon Punctuation indicates reading frame. |
Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI, HincII, NcoI and NotI/XhoI. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by W. De Schamphelaire(1) and Prof. Dr R. Beyaert(1). (1) BCCM/GeneCorner, Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
Plasmid reference: | - |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 MC1061 |
Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Cultivation remark: | - |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pCAGGS-E-pipB (LMBP 6731) is available at BCCM/GeneCorner. This plasmid was deposited by W. De Schamphelaire and Prof. Dr R. Beyaert. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.