GREAT AT SMALL THINGS

0

GeneCorner plasmid details

Last data update: 24 October 2020 04:12 CEST

Plasmid name: pCAGGS-E-sciL (LMBP 8988)

Add to cart

New search

Price category: Cat. 2 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p8988.gb
Sequence
analysis results
Genecorner:

Sanger: 104809.fasta

Sanger: 104808.fasta

Cloned DNA: Salmonella enterica serovar Typhimurium LT2 sciL cDNA
E-tag; N-terminal
Promoter: Chicken β-actin/rabbit β-globin hybrid promoter (AG)
Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only
Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: Rabbit β-globin polyadenylation signal (β-globin polyA)
Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Parental clone: pCAGGSEhA20
Further information: The plasmid was constructed by replacing the hA20 coding sequence from the NotI/XhoI opened pCAGGSEhA20 by the PCR amplified S. enterica sciL coding sequence.
Protein expression of this vector in HEK293T cells was tested at GeneCorner.
The region of the sciL coding sequence was sequenced by GeneCorner.
The nucleotide sequence of the S. enterica sciL coding sequence corresponds with the EMBL Nucleotide Sequence Database accession number AE006468.1.
EMBL Accession number: AE006468.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 20/03/2017
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr M. De Ceuleneer(1) and Prof. Dr R. Beyaert(1)(2).
(1) BCCM/GeneCorner, Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
(2) VIB Center for Inflammation Research, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pCAGGS-E-sciL (LMBP 8988) is available at BCCM/GeneCorner. This plasmid was deposited by Dr M. De Ceuleneer and Prof. Dr R. Beyaert.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

New search