Last data update: 11 June 2021 14:12 CEST
Plasmid name: pCAGGS-F-V278A-Q279A (LMBP 10255)
|New search||Print data sheet|
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
Human respiratory syncitial virus synthetic fusion protein cDNA (F); mutated sequence|
|Promoter:||Chicken β-actin/rabbit β-globin hybrid promoter (AG)
Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only
Escherichia coli lac operon promoter
|Terminator:||Rabbit β-globin polyadenylation signal (β-globin polyA)
Simian virus 40 polyadenylation signal (SV40 polyA)
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin
Simian virus 40 bidirectional origin (SV40)
|Host range:||Escherichia coli
Mammalian cells; SV40 permissive cells
|Further information:||The plasmid was constructed by cloning the mutated sequence of the human RSV fusion protein into the EcoRI/BglII opened pCAGGS vector.
The human RSV fusion protein contains V278A and Q279A mutations.
The nucleotide sequence of the parental pCAGGS vector corresponds with the EMBL Nucleotide Sequence Database accession number LT727518.1.
Other name of the plasmid is pCAGGS Fmutant (VQ-AA).
|EMBL Accession number:||LT727518.1, view at EMBL, GenBank, DDBJ|
|Latest sequence update:||17/08/2018|
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by Prof. Dr X. Saelens(1)(2)(3). It was constructed by L. Vande Ginste(1)(2)(3).
(1) Department of Medical Protein Research, VIB, Ghent, Belgium
(2) Inflammation Research Center, VIB, Ghent, Belgium
(3) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Restricted distribution:||- BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 DH5α|
|Host reference:||Focus 8 (1986), 9
|Related host reference:||Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pCAGGS-F-V278A-Q279A (LMBP 10255) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr X. Saelens.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.