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GeneCorner plasmid details

Last data update: 27 October 2020 04:27 CET

Plasmid name: pCAGGS-FLAG-DUBA (LMBP 6414)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p6414.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human OTU domain containing 5 cDNA (OTUD5, DUBA); isoform b (lacks GTSAV)
FLAG epitope tag; N-terminal
Promoter: Chicken β-actin/rabbit β-globin hybrid promoter (AG)
Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only
Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: Rabbit β-globin polyadenylation signal (β-globin polyA)
Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Parental clone: pCAGGS-mA20
Further information: The plasmid was constructed by inserting a PCR fragment, containing the coding sequence of the hDUBA isoform b, between the KpnI and SalI sites of pCAGGS-mA20. Isoform b of hDUBA lacks the 5 amino acids GTSAV in the OTU domain, compared to isoform a.
The gene hDUBA encodes a member of the OTU (ovarian tumor) domain-containing cysteine proteases.
The OTU domain confers deubiquitinase activity and the encoded protein has been shown to suppress the type I interferon-dependent innate immune response by cleaving the polyubiquitin chain from an essential type I interferon adaptor protein. Cleavage results in disassociation of the adaptor protein from a downstream signaling complex and disruption of the type I interferon signaling cascade.
Other name of the plasmid is pCAGGS-flag-DUBA.
EMBL Accession number: NM_001136158, view at GenBank
Latest sequence update: 01/07/2013
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr R. Beyaert(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Related plasmid reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Verhelst er al., Biochem. Biophys. Res. Commun. 465 (2015), 275-280 [PMID: 26260321] [DOI: 10.1016/j.bbrc.2015.08.019]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pCAGGS-FLAG-DUBA (LMBP 6414) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Beyaert .

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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