Last data update: 27 October 2020 04:27 CET
Plasmid name: pCAGGS-FLAG-DUBA (LMBP 6414)
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p6414.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: | Human OTU domain containing 5 cDNA (OTUD5, DUBA); isoform b (lacks GTSAV) FLAG epitope tag; N-terminal |
| Promoter: | Chicken β-actin/rabbit β-globin hybrid promoter (AG) Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Rabbit β-globin polyadenylation signal (β-globin polyA) Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pCAGGS-mA20 |
| Further information: | The plasmid was constructed by inserting a PCR fragment, containing the coding sequence of the hDUBA isoform b, between the KpnI and SalI sites of pCAGGS-mA20. Isoform b of hDUBA lacks the 5 amino acids GTSAV in the OTU domain, compared to isoform a. The gene hDUBA encodes a member of the OTU (ovarian tumor) domain-containing cysteine proteases. The OTU domain confers deubiquitinase activity and the encoded protein has been shown to suppress the type I interferon-dependent innate immune response by cleaving the polyubiquitin chain from an essential type I interferon adaptor protein. Cleavage results in disassociation of the adaptor protein from a downstream signaling complex and disruption of the type I interferon signaling cascade. Other name of the plasmid is pCAGGS-flag-DUBA. |
| EMBL Accession number: | NM_001136158, view at GenBank |
| Latest sequence update: | 01/07/2013 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr R. Beyaert(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Related plasmid reference: | Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] Verhelst er al., Biochem. Biophys. Res. Commun. 465 (2015), 275-280 [PMID: 26260321] [DOI: 10.1016/j.bbrc.2015.08.019] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
|
Refer in your Materials and Methods: |
pCAGGS-FLAG-DUBA (LMBP 6414) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Beyaert . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.